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  4. Integrin Associated Protein/CD47
  5. CD47 Protein, Human (Biotinylated, HEK293, Avi-His)

CD47 Protein, Human (Biotinylated, HEK293, Avi-His)

Cat. No.: HY-P72359
COA Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
20 μg $455 In-stock
50 μg $850 In-stock
100 μg $1365 In-stock
250 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

1.Immobilized Biotinylated Human CD47-His at 2 μg/mL (100 μl/well). Dose response curve for Human SIRP alpha-hFc with the EC50 of 0.03 ng/mL determined by ELISA.
2.Immobilized Human SIRP alpha, hFc Tag at 1 μg/mL (100 μl/well) on the plate. Dose responsecurve for Biotinylated Human CD47, His Tag with the EC50 of< 0.48 μg/mL determined by ELISA








Q08722 (Q19-P139)

Gene ID

961  [NCBI]

Leukocyte Surface Antigen CD47; Antigenic Surface Determinant Protein OA3; IAP; MER6
AA Sequence


Molecular Weight

30-55 kDa


Greater than 95% as determined by reducing SDS-PAGE or Tis-Bis PAGE.


Lyophilized powder.


Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-Citrate, 150 mM NaCl, pH 8.0 or PBS (pH 7.4), normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.


It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.


Room temperature in continental US; may vary elsewhere.

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD47 Protein, Human (Biotinylated, HEK293, Avi-His)
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