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CD73/5'-Nucleotidase Protein, Mouse (HEK293, His)

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CD73/5'-Nucleotidase Protein Protein, Mouse (HEK293, His) is a recombinant mouse CD73 expressed by HEK293 cells with His tag. CD73 is a cell surface enzyme that catalyzes the dephosphorylation of extracellular AMP to adenosine.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for CD73/5'-Nucleotidase Protein, Mouse (HEK293, His)
  • CD73/5'-Nucleotidase Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

CD73/5'-Nucleotidase Protein Protein, Mouse (HEK293, His) is a recombinant mouse CD73 expressed by HEK293 cells with His tag. CD73 is a cell surface enzyme that catalyzes the dephosphorylation of extracellular AMP to adenosine[1][2].

Background

CD73 is a glycosyl-phosphatidylinositol (GPI)-linked 70-kDa cell surface enzyme found in most tissues. CD73, originally defined as a lymphocyte differentiation antigen, is thought to function as a co-signaling molecule on T lymphocytes and as an adhesion molecule that is important for lymphocyte binding to endothelium[2].

Verified Bioactivity

Measured by its ability to hydrolyze the 5’-phosphate group from 100μM substrate adenosine-5’-monophosphate (AMP) (HY-W010968). The specific activity is 17688.25 pmol/min/µg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q61503 (W29-F550)

  • Gene ID
    23959  [NCBI]
  • Molecular Construction
    • N-term
    • CD73 (W29-F550)
      Accession # Q61503
    • 6*His
    • C-term
  • Protein Length

    Full Length of 5'-nucleotidase Chain

  • Synonyms

    NT5E; ENT; Prev. NT5; 5'-NT; IMP-Specific 5'-Nucleotidase; NTE; Thymidylate 5'-Phosphatase; 5'-Nucleotidase, Ecto (CD73); 5'-Deoxynucleotidase; Purine 5-Prime-Nucleotidase; Ecto-5'-Nucleotidase; 5' Nucleotidase (CD73); 5'-Nucleotidase; CD73 Antigen; CALJA

  • AA Sequence

    WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKF

  • Predicted Molecular Mass

    58.8 kDa

  • Molecular Weight

    Approximately 60 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Bin Zhang. CD73 promotes tumor growth and metastasis. Oncoimmunology. 2012 Jan 1; 1(1): 67–70. [Content Brief]

[2]. Bin Zhang. CD73: A novel target for cancer immunotherapy. Cancer Res. 2010 Aug 15; 70(16): 6407–6411. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • CD73/5'-Nucleotidase Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CD73/5'-Nucleotidase Protein, Mouse (HEK293, His)
  • CD73/5'-Nucleotidase Protein, Mouse (HEK293, His) Product Image