CD73/5'-Nucleotidase Protein, Mouse (HEK293, His)

CD73/5'-Nucleotidase Protein Protein, Mouse (HEK293, His) is a recombinant mouse CD73 expressed by HEK293 cells with His tag. CD73 is a cell surface enzyme that catalyzes the dephosphorylation of extracellular AMP to adenosine^[1][2].

CD73 is a glycosyl-phosphatidylinositol (GPI)-linked 70-kDa cell surface enzyme found in most tissues. CD73, originally defined as a lymphocyte differentiation antigen, is thought to function as a co-signaling molecule on T lymphocytes and as an adhesion molecule that is important for lymphocyte binding to endothelium^[2].

Mouse

HEK293

C-6*His

Q61503 (W29-F550)

23959  [NCBI]

WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKF

Approximately 60.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 120mM NaCl, 4 mM CaCl₂, 20% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Bin Zhang. CD73 promotes tumor growth and metastasis. Oncoimmunology. 2012 Jan 1; 1(1): 67–70.  [Content Brief]

  [2]. Bin Zhang. CD73: A novel target for cancer immunotherapy. Cancer Res. 2010 Aug 15; 70(16): 6407–6411.  [Content Brief]