CD73/5'-Nucleotidase Protein, Rat (HEK293, His)

CD73/5'-Nucleotidase Protein, Rat (HEK293, His) is a 70-90 kDa rat CD73 protein with His-flag expressed by HEK293. Rat CD73 is a cell membrane protein that catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides^[1].

CD-73, also known as NT5E, 5'-nucleotidase, and CD73 is a cell membrane protein which belongs to the5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol-anchored protein with both ecto-enzyme activity (ecto-5'-nucleotidase) and serves as a costimulatory molecule in activating T cells. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides^[1].

Rat

HEK293

C-6*His

P21588 (W29-K549)

58813  [NCBI]

Partial

WELTIMHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLLLDAGDQYQGTIWFTVYKGLEVAHFMNLLGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAPQISGLYLPYKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEVTALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHTNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKVEFDDKGNVVTSYGNPILLNSTIREDAAIKADINQWRIKLDNYSTQELGRTIVYLNGSAQECRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDISRKPWDRVVQLKVLCTKCRVPIYEPLEMDKVYKVVLPSYLVNGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVIYPAVEGRIK

70-90 kDa

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Cynthia St Hilaire, et al. NT5E mutations and arterial calcifications  [Content Brief]

  [2]. R Resta, et al. T cell signalling through CD73. Cell Signal. 1997 Feb;9(2):131-9.  [Content Brief]

  [3]. Y Yamashita, et al. CD73 expression and fyn-dependent signaling on murine lymphocytes.Eur J Immunol. 1998 Oct;28(10):2981-90.  [Content Brief]