CD73/5'-Nucleotidase Protein, Rat (HEK293, His)

CD73/5'-Nucleotidase Protein, Rat (HEK293, His) is a 70-90 kDa rat CD73 protein with His-flag expressed by HEK293. Rat CD73 is a cell membrane protein that catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides^[1].

CD-73, also known as NT5E, 5'-nucleotidase, and CD73 is a cell membrane protein which belongs to the5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol-anchored protein with both ecto-enzyme activity (ecto-5'-nucleotidase) and serves as a costimulatory molecule in activating T cells. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides^[1].

Rat

HEK293

C-6*His

P21588 (W29-K549)

58813  [NCBI]

WELTIMHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLLLDAGDQYQGTIWFTVYKGLEVAHFMNLLGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAPQISGLYLPYKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEVTALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHTNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKVEFDDKGNVVTSYGNPILLNSTIREDAAIKADINQWRIKLDNYSTQELGRTIVYLNGSAQECRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDISRKPWDRVVQLKVLCTKCRVPIYEPLEMDKVYKVVLPSYLVNGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVIYPAVEGRIKHHHHHH

70-90 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 120mM NaCl, 4 mM CaCl₂, 20% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Cynthia St Hilaire, et al. NT5E mutations and arterial calcifications  [Content Brief]

  [2]. R Resta, et al. T cell signalling through CD73. Cell Signal. 1997 Feb;9(2):131-9.  [Content Brief]

  [3]. Y Yamashita, et al. CD73 expression and fyn-dependent signaling on murine lymphocytes.Eur J Immunol. 1998 Oct;28(10):2981-90.  [Content Brief]