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  5. CD8 alpha Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)

CD8 alpha Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P705292
Handling Instructions Technical Support

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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25 μg Get quote 3 - 4 Weeks 4 - 5 Weeks 5 - 6 Weeks 2 - 3 weeks
200 μg Get quote 3 - 4 Weeks 4 - 5 Weeks 5 - 6 Weeks 2 - 3 weeks
> 200 μg   Get quote  
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Cynomolgus CD8 alpha Protein, His,Avi tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-CD8 alpha Antibody, Human IgG1 with a linear range of 0.06-1 ng/mL.

Species

Cynomolgus

Source

HEK293

Tag

C-Avi;C-His

Accession

XP_005575419 (N63-D223)

Gene ID

102124337

Molecular Construction
N-term
(M42-D223)
Accession # XP_005575419
Avi-His
C-term
Conjugation

Biotin

Synonyms
T-cell surface glycoprotein CD8 alpha chain; CD8a; CD8A; MAL
AA Sequence

NQFRVSPLGRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGTAARPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLRDFRQENEGYYFCSALSNSIMYFSRFVPVFLPAKPTTTPAPRPPTPAPTTASQPLSLRPEACRPAAGGSVNTRGLDFACD

Predicted Molecular Mass
21.3 kDa.
Molecular Weight

Approximately 30-33 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Glycosylation
Yes
Purity

≥ 90%, as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from 0.22 μm filtered solution of PBS, pH 7.4 with 10% trehalose as protectant.

Endotoxin Level

/

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

CD8 alpha Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD8 alpha Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)

 

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Product Name:
CD8 alpha Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P705292
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