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CD97 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

CD97, a receptor crucial for leukocyte migration, participates in adhesion and signaling during early leukocyte activation. Forming a heterodimer with a large extracellular alpha subunit and a seven-transmembrane beta subunit, CD97 interacts with complement decay-accelerating factor (DAF). The largest isoform (isoform 1) specifically engages with chondroitin sulfate, showcasing its diverse roles in cellular processes. CD97 Protein, Human (HEK293, His) is the recombinant human-derived CD97 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CD97 Protein, Human (HEK293, His)
  • Experimental Validation Results for CD97 Protein, Human (HEK293, His)
  • CD97 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

CD97, a receptor crucial for leukocyte migration, participates in adhesion and signaling during early leukocyte activation. Forming a heterodimer with a large extracellular alpha subunit and a seven-transmembrane beta subunit, CD97 interacts with complement decay-accelerating factor (DAF). The largest isoform (isoform 1) specifically engages with chondroitin sulfate, showcasing its diverse roles in cellular processes. CD97 Protein, Human (HEK293, His) is the recombinant human-derived CD97 protein, expressed by HEK293 , with C-His labeled tag.

Background

CD97, a receptor with potential involvement in both adhesion and signaling processes during early leukocyte activation, plays a crucial role in facilitating leukocyte migration. This receptor forms a heterodimer, comprising a large extracellular alpha subunit non-covalently linked to a seven-transmembrane beta subunit. CD97 interacts with complement decay-accelerating factor (DAF) and the largest isoform (isoform 1) specifically engages with chondroitin sulfate, highlighting its diverse interactions in cellular processes.

Verified Bioactivity

Measured by it's binding ability in a functional ELISA. Immobilized human CD55 at 2 μg/ml (100 μl/well) can bind Biotinylated human CD97. The ED50 for this effect is 14.12 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    P48960-2/NP_001775.2 (Q21-Q398)

  • Gene ID
    976  [NCBI]
  • Molecular Construction
    • N-term
    • CD97 (Q21-Q398)
      Accession # P48960-2/NP_001775.2
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    ADGRE5; Seven-Transmembrane, Heterodimeric Receptor Associated With Inflammation; Prev. CD97; Seven-Span Transmembrane Protein; Leukocyte Antigen CD97; CD97 Molecule; TM7LN1; CD97 Antigen; Adhesion G Protein-Coupled Receptor E5; Seven Transmembrane Helix

  • AA Sequence

    QDSRGCARWCPQNSSCVNATACRCNPGFSSFSEIITTPTETCDDINECATPSKVSCGKFSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESENTCQDVDECSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMTFSTWTPPPGVHSQTLSRFFDKVQDLGRDSKTSSAEVTIQNVIKLVDELMEAPGDVEALAPPVRHLIATQLLSNLEDIMRILAKSLPKGPFTYISPSNTELTLMIQERGDKNVTMGQSSARMKLNWAVAAGAEDPGPAVAGILSIQNMTTLLANASLNLHSKKQAELEEIYESSIRGVQLRRLSAVNSIFLSHNNTKELNSPILFAFSHLESSDGEAGRDPPAKDVMPGPRQ

  • Molecular Weight

    Approximately 60-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE. The protein migrates as a 60-82 kDa band under reducing SDS-PAGE due to glycosylation.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized human CD55 at 2 μg/mL (100 μl/well) can bind Biotinylated human CD97. The ED50 for this effect is 14.12 ng/mL.

  • CD97 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CD97 Protein, Human (HEK293, His)
  • Experimental Validation Results for CD97 Protein, Human (HEK293, His)
  • CD97 Protein, Human (HEK293, His) Product Image