CD97 Protein, Human (HEK293, His)

CD97, a receptor crucial for leukocyte migration, participates in adhesion and signaling during early leukocyte activation. Forming a heterodimer with a large extracellular alpha subunit and a seven-transmembrane beta subunit, CD97 interacts with complement decay-accelerating factor (DAF). The largest isoform (isoform 1) specifically engages with chondroitin sulfate, showcasing its diverse roles in cellular processes. CD97 Protein, Human (HEK293, His) is the recombinant human-derived CD97 protein, expressed by HEK293 , with C-His labeled tag.

CD97, a receptor with potential involvement in both adhesion and signaling processes during early leukocyte activation, plays a crucial role in facilitating leukocyte migration. This receptor forms a heterodimer, comprising a large extracellular alpha subunit non-covalently linked to a seven-transmembrane beta subunit. CD97 interacts with complement decay-accelerating factor (DAF) and the largest isoform (isoform 1) specifically engages with chondroitin sulfate, highlighting its diverse interactions in cellular processes.

Measured by it's binding ability in a functional ELISA. Immobilized human CD55 at 2 μg/ml (100 μl/well) can bind Biotinylated human CD97. The ED₅₀ for this effect is 14.12 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized human CD55 at 2 μg/mL (100 μl/well) can bind Biotinylated human CD97. The ED₅₀ for this effect is 14.12 ng/mL.

Human

HEK293

C-His

P48960-2/NP_001775.2 (Q21-Q398)

976  [NCBI]

Partial

QDSRGCARWCPQNSSCVNATACRCNPGFSSFSEIITTPTETCDDINECATPSKVSCGKFSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESENTCQDVDECSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMTFSTWTPPPGVHSQTLSRFFDKVQDLGRDSKTSSAEVTIQNVIKLVDELMEAPGDVEALAPPVRHLIATQLLSNLEDIMRILAKSLPKGPFTYISPSNTELTLMIQERGDKNVTMGQSSARMKLNWAVAAGAEDPGPAVAGILSIQNMTTLLANASLNLHSKKQAELEEIYESSIRGVQLRRLSAVNSIFLSHNNTKELNSPILFAFSHLESSDGEAGRDPPAKDVMPGPRQ

60-82 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE. The protein migrates as a 60-82 kDa band under reducing SDS-PAGE due to glycosylation.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.