Copy Link Print Information

Cerebellin-2 Protein, Human (CHO, HA)

Customer Review

Based on 1 Customer Validation

The protein Cerebellin-2 organizes synapses in specific brain neurons, which is critical for long-term maintenance. It forms homohexamers composed of disulfide-bonded homotrimers connected through the C1q domain. Cerebellin-2 Protein, Human (CHO, HA) is the recombinant human-derived Cerebellin-2 protein, expressed by CHO , with N-HA labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: CHO
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Cerebellin-2 Protein, Human (CHO, HA)
  • Experimental Validation Results for Cerebellin-2 Protein, Human (CHO, HA)
  • Cerebellin-2 Protein, Human (CHO, HA) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The protein Cerebellin-2 organizes synapses in specific brain neurons, which is critical for long-term maintenance. It forms homohexamers composed of disulfide-bonded homotrimers connected through the C1q domain. Cerebellin-2 Protein, Human (CHO, HA) is the recombinant human-derived Cerebellin-2 protein, expressed by CHO , with N-HA labeled tag.

Background

Cerebellin-2 Protein serves as a synaptic organizer in specific neuronal subsets in the brain, playing an essential role in the long-term maintenance, though not the establishment, of excitatory synapses. It forms homohexamers linked by disulfide-bound homotrimers, assembled through the globular C1q domains, with the trimers associating via N-terminal cysteine residues to create disulfide-linked hexamers. Cerebellin-2 may also form homooligomers or heterooligomers with CBLN1 and CBLN3 before secretion. Once secreted, it no longer interacts with other CBLN family members. Cerebellin-2 engages in interactions with GRID2, and to a lesser extent with GRID1. Additionally, it interacts with NRXN1 and NRXN2 long and short isoforms generated through alternative promoter usage. The protein exhibits weak interaction with NRXN3 short isoform and no interaction with NRXN3 long isoform.

Verified Bioactivity

Measured in a cell proliferation assay using SH-SY5Y cells. The ED50 for this effect is 1.222 μg/mL, corresponding to a specific activity is 818.331 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a cell proliferation assay using SH-SY5Y cells. The ED50 for this effect is 1.222 μg/mL, corresponding to a specific activity is 818.331 units/mg.

Technical Parameters

  • Species Human
  • Source CHO
  • Tag N-HA
  • Accession

    Q8IUK8 (R50-L224)

  • Gene ID
    147381  [NCBI]
  • Molecular Construction
    • N-term
    • HA
    • Cerebellin-2 (R50-L224)
      Accession # Q8IUK8
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CBLN2; Cerebellin-2; Cerebellin 2 Precursor

  • AA Sequence

    RAQNDTEPIVLEGKCLVVCDSSPSADGAVTSSLGISVRSGSAKVAFSATRSTNHEPSEMSNRTMTIYFDQVLVNIGNHFDLASSIFVAPRKGIYSFSFHVVKVYNRQTIQVSLMQNGYPVISAFAGDQDVTREAASNGVLLLMEREDKVHLKLERGNLMGGWKYSTFSGFLVFPL

  • Predicted Molecular Mass

    20.2 kDa

  • Molecular Weight

    Approximately 30 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 200 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using SH-SY5Y cells. The ED50 for this effect is 1.222 μg/mL, corresponding to a specific activity is 818.331 units/mg.

  • Cerebellin-2 Protein, Human (CHO, HA) Product Image
    1/16
  • Experimental Validation Results for Cerebellin-2 Protein, Human (CHO, HA)
  • Experimental Validation Results for Cerebellin-2 Protein, Human (CHO, HA)
  • Cerebellin-2 Protein, Human (CHO, HA) Product Image