CRBN Protein, Human (HEK293, Flag)

CRBN Protein, Human (HEK293, Flag) is the recombinant human-derived Cereblon, expressed by HEK293 , with N-3*Flag labeled tag.

CRBN serves as the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, mediating ubiquitination and subsequent proteasomal degradation of target proteins like MEIS2, ILF2, or GLUL. Its proper degradation of key regulatory proteins is essential for normal limb outgrowth and fibroblast growth factor FGF8 expression. CRBN maintains presynaptic glutamate release and cognitive functions (e.g., memory and learning) by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. It likely functions by modulating BK channel assembly and neuronal surface expression via interaction with KCNT1. By similarity, CRBN may also regulate anxiety-like behaviors through a BK channel-independent mechanism. In TLR4 signaling, CRBN acts as a negative regulator by binding TRAF6 and ECSIT, thereby inhibiting ECSIT ubiquitination—a critical step in the pathway.

Immobilized Lenalidomide (HY-A0003) at 20 μg/mL (100 μL/well) can bind Biotinylated Human CRBN. The ED₅₀ for this effect is 216 ng/mL.

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  Immobilized Lenalidomide (HY-A0003) at 20 μg/mL (100 μL/well) can bind Biotinylated Human CRBN. The ED₅₀ for this effect is 216 ng/mL.

Human

HEK293

N-3*Flag

Q96SW2-1 (A2-L442)

51185

Full Length of Isoform 1

AGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL

Approximately 60-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  ≥ 85%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution in 40 mM Tris-HCl, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, pH 8.0, 20% Glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.