Chk1 Protein, Human (sf9, GST)

Chk1 Protein, the subject of the paragraph, is not mentioned in the provided text. If you would like a summary involving Chk1, please provide the relevant paragraph or information about Chk1. Chk1 Protein, Human (sf9, GST) is the recombinant human-derived Chk1 protein, expressed by Sf9 insect cells, with N-GST labeled tag. The total length of Chk1 Protein, Human (sf9, GST) is 476 a.a., with molecular weight of ~80 kDa.

The given text describes the function of a protein known as CDC25. CDC25 is responsible for regulating the cell cycle progression by phosphorylating and activating cyclin-dependent kinase (CDK)-cyclin complexes. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes, which blocks cell cycle progression.

The specific activity was determined to be > 15 nmol/min/mg using CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.

Human

Sf9 insect cells

N-GST

AAM78553.1 (M1-T476)

1111  [NCBI]

Approximately 80 kDa

Greater than 85% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol, 2 mM GSH.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.