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  5. Coagulation Factor VII/F7 Protein, Mouse (CHO, His)

Coagulation Factor VII/F7 Protein, Mouse (CHO, His)

Cat. No.: HY-P73045
COA Handling Instructions

Coagulation Factor VII (F7) protein lacks crucial conserved residue(s) needed for propagating feature annotation. Coagulation Factor VII/F7 Protein, Mouse (CHO, His) is the recombinant mouse-derived Coagulation Factor VII/F7 protein, expressed by CHO , with C-His labeled tag. The total length of Coagulation Factor VII/F7 Protein, Mouse (CHO, His) is 422 a.a., with molecular weight of 56-63 kDa.

For research use only. We do not sell to patients.

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Description

Coagulation Factor VII (F7) protein lacks crucial conserved residue(s) needed for propagating feature annotation. Coagulation Factor VII/F7 Protein, Mouse (CHO, His) is the recombinant mouse-derived Coagulation Factor VII/F7 protein, expressed by CHO , with C-His labeled tag. The total length of Coagulation Factor VII/F7 Protein, Mouse (CHO, His) is 422 a.a., with molecular weight of 56-63 kDa.
Background

Coagulation Factor VII (F7) lacks conserved residue(s) required for the propagation of feature annotation.
Biological Activity

Measured by its binding ability in a functional ELISA.Immobilized mouse F7-his at 10 μg/mL (100 μl/well) can bind biotinylated mouse F3-his. The EC50 of biotinylated mouse F3-his is 0.1-0.3 μg/mL.
Species

Mouse
Source

CHO
Tag

C-His
Accession

Q542C2 (V25-L446)
Gene ID

14068  [NCBI]
Molecular Construction
N-term
F7 (V25-L446)
Accession # Q542C2
His
C-term
Synonyms
Coagulation factor VII; Eptacog alfa; F7; FVII coagulation protein; SPCA
AA Sequence

MVPQAHGLLLLCFLLQLQGPLGTAVFITQEEAHGVLHRQRRANSLLEELWPGSLERECNEEQCSFEEAREIFKSPERTKQFWIVYSDGDQCASNPCQNGGTCQDHLKSYVCFCLLDFEGRNCEKSKNEQLICANENGDCDQYCRDHVGTKRTCSCHEDYTLQPDEVSCKPKVEYPCGRIPVVEKRNSSSRQGRIVGGNVCPKGECPWQAVLKINGLLLCGAVLLDARWIVTAAHCFDNIRYWGNITVVMGEHDFSEKDGDEQVRRVTQVIMPDKYIRGKINHDIALLRLHRPVTFTDYVVPLCLPEKSFSENTLARIRFSRVSGWGQLLDRGATALELMSIEVPRLMTQDCLEHAKHSSNTPKITENMFCAGYMDGTKDACKGDSGGPHATHYHGTWYLTGVVSWGEGCAAIGHIGVYTRVSQYIDWLVRHMDSKLQVGVFRLPLL

Molecular Weight

56-63 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Appearance

Solution.
Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation

COA (219 KB)

Handling Instructions (2659 KB)

Coagulation Factor VII/F7 Protein, Mouse (CHO, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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