CROT Protein, Human (V474L, sf9, His)
CROT protein is critical in the β-oxidation process, showing the highest enzymatic activity on C6 to C10 substrates. As a key player in lipid metabolism, CROT promotes the conversion of 4,8-dimethylnonanoyl-CoA to its carnitine ester, regulating fatty acid oxidation to achieve cellular energy balance. CROT Protein, Human (V474L, sf9, His) is the recombinant human-derived CROT protein, expressed by Sf9 insect cells , with C-His labeled tag and V474L mutation.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
CROT protein is critical in the β-oxidation process, showing the highest enzymatic activity on C6 to C10 substrates. As a key player in lipid metabolism, CROT promotes the conversion of 4,8-dimethylnonanoyl-CoA to its carnitine ester, regulating fatty acid oxidation to achieve cellular energy balance. CROT Protein, Human (V474L, sf9, His) is the recombinant human-derived CROT protein, expressed by Sf9 insect cells , with C-His labeled tag and V474L mutation.
Background
The CROT Protein serves a pivotal role in the beta-oxidation of fatty acids, exhibiting the highest enzymatic activity toward substrates with chain lengths ranging from C6 to C10. Functioning as a key player in lipid metabolism, CROT facilitates the conversion of the end product of pristanic acid beta-oxidation, namely 4,8-dimethylnonanoyl-CoA, into its corresponding carnitine ester. This enzymatic process is essential in regulating the balance of fatty acid oxidation, contributing to cellular energy homeostasis. Understanding the specific activities of CROT provides insights into the intricate mechanisms governing fatty acid catabolism, with potential implications for metabolic regulation and energy production. Further investigation into the function of CROT holds promise for advancing our understanding of lipid metabolism and may offer avenues for therapeutic interventions in metabolic disorders.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag C-His
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Accession
Q9UKG9-1 (M1-L612, V474L)
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Molecular Construction
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N-term
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CROT (M1-L612,V474L)
Accession # Q9UKG9-1 -
His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
CROT; Peroxisomal Carnitine O-Octanoyltransferase; Carnitine O-Octanoyltransferase; Peroxisomal Carnitine Acyltransferase; COT
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AA Sequence
MENQLAKSTEERTFQYQDSLPSLPVPSLEESLKKYLESVKPFANQEEYKKTEEIVQKFQSGIGEKLHQKLLERAKGKRNWLEEWWLNVAYLDVRIPSQLNVNFAGPAAHFEHYWPPKEGTQLERGSITLWHNLNYWQLLRKEKVPVHKVGNTPLDMNQFRMLFSTCKVPGITRDSIMNYFRTESEGRSPNHIVVLCRGRAFVFDVIHEGCLVTPPELLRQLTYIHKKCHSEPDGPGIAALTSEERTRWAKAREYLIGLDPENLALLEKIQSSLLVYSMEDSSPHVTPEDYSEIIAAILIGDPTVRWGDKSYNLISFSNGVFGCNCDHAPFDAMIMVNISYYVDEKIFQNEGRWKGSEKVRDIPLPEELIFIVDEKVLNDINQAKAQYLREASDLQIAAYAFTSFGKKLTKNKMLHPDTFIQLALQLAYYRLHGHPGCCYETAMTRHFYHGRTETMRSCTVEAVRWCQSMQDPSLNLRERQQKMLQAFAKHNKMMKDCSAGKGFDRHLLGLLLIAKEEGLPVPELFTDPLFSKSGGGGNFVLSTSLVGYLRVQGVVVPMVHNGYGFFYHIRDDRFVVACSAWKSCPETDAEKLVQLTFCAFHDMIQLMNSTHL
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Predicted Molecular Mass
71.5 kDa
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Molecular Weight
Approximately 65 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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