CROT Protein, Human (V474L, sf9, His)

CROT Protein is pivotal in beta-oxidation, displaying highest enzymatic activity on C6 to C10 substrates. As a key player in lipid metabolism, CROT facilitates the conversion of 4,8-dimethylnonanoyl-CoA to its carnitine ester, regulating fatty acid oxidation for cellular energy balance. Understanding CROT's specific activities unveils mechanisms governing fatty acid catabolism, with potential implications for metabolic regulation and energy production. Further investigation into CROT's function holds promise for therapeutic interventions in metabolic disorders. CROT Protein, Human (V474L, sf9, His) is the recombinant human-derived CROT protein, expressed by Sf9 insect cells , with C-His labeled tag and V474L mutation. The total length of CROT Protein, Human (V474L, sf9, His) is 612 a.a., with molecular weight of ~65 kDa.

The CROT Protein serves a pivotal role in the beta-oxidation of fatty acids, exhibiting the highest enzymatic activity toward substrates with chain lengths ranging from C6 to C10. Functioning as a key player in lipid metabolism, CROT facilitates the conversion of the end product of pristanic acid beta-oxidation, namely 4,8-dimethylnonanoyl-CoA, into its corresponding carnitine ester. This enzymatic process is essential in regulating the balance of fatty acid oxidation, contributing to cellular energy homeostasis. Understanding the specific activities of CROT provides insights into the intricate mechanisms governing fatty acid catabolism, with potential implications for metabolic regulation and energy production. Further investigation into the function of CROT holds promise for advancing our understanding of lipid metabolism and may offer avenues for therapeutic interventions in metabolic disorders.

Human

Sf9 insect cells

C-His

Q9UKG9 (M1-L612,V474L)

54677  [NCBI]

Approximately 65 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.