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CTRC Protein, Human (HEK293, His)

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Based on 1 Customer Validation

CTRC proteins critically regulate trypsinogen and procarboxypeptidase, precisely cleaving their zymogen precursors to influence activation and degradation. Its chymotrypsin-type protease activity helps control enzyme processing. CTRC Protein, Human (HEK293, His) is the recombinant human-derived CTRC protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for CTRC Protein, Human (HEK293, His)
  • CTRC Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

CTRC proteins critically regulate trypsinogen and procarboxypeptidase, precisely cleaving their zymogen precursors to influence activation and degradation. Its chymotrypsin-type protease activity helps control enzyme processing. CTRC Protein, Human (HEK293, His) is the recombinant human-derived CTRC protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The CTRC protein plays a crucial role in the regulation of trypsinogens and procarboxypeptidases by precisely targeting specific cleavage sites within their zymogen precursors, thereby influencing their activation and degradation processes. With its chymotrypsin-type protease activity, CTRC contributes to the controlled processing of these enzymes. Additionally, it exhibits hypocalcemic activity, suggesting its involvement in calcium homeostasis or signaling pathways. The multifaceted functions of CTRC underscore its significance in modulating key proteolytic events, with implications for various physiological processes and potential relevance in pathological conditions.

Verified Bioactivity

Measured by its ability to cleave 100 μM peptide substrate Suc-AAPF-AMC that incubate at 37 °C for 1 hour in kinetic mode for 5 minutes. The specific activity is 855.3072 pmol/min/μg. (Activation description: The proenzyme needs to be activated by Trypsin for an activated form.)

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q99895 (C17-L268)

  • Gene ID
    11330  [NCBI]
  • Molecular Construction
    • N-term
    • CTRC (C17-L268)
      Accession # Q99895
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CTRC; Elastase 4; Chymotrypsin C; Uncharacterized Protein DKFZp779K0837; CLCR; Serum Calcium Decreasing Factor; Caldecrin; Chymotrypsin C (Caldecrin); ELA4; DKFZp779K0837; Chymotrypsinogen C; Elastase IV; Chymotrypsin-C

  • AA Sequence

    CGVPSFPPNLSARVVGGEDARPHSWPWQISLQYLKNDTWRHTCGGTLIASNFVLTAAHCISNTRTYRVAVGKNNLEVEDEEGSLFVGVDTIHVHKRWNALLLRNDIALIKLAEHVELSDTIQVACLPEKDSLLPKDYPCYVTGWGRLWTNGPIADKLQQGLQPVVDHATCSRIDWWGFRVKKTMVCAGGDGVISACNGDSGGPLNCQLENGSWEVFGIVSFGSRRGCNTRKKPVVYTRVSAYIDWINEKMQL

  • Predicted Molecular Mass

    29 kDa

  • Molecular Weight

    Approximately 33-40 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • CTRC Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CTRC Protein, Human (HEK293, His)
  • CTRC Protein, Human (HEK293, His) Product Image