DAPK3/ZIPK Protein, Human (sf9, GST)

The DAPK3/ZIPK protein is a multifunctional serine/threonine kinase that complexly regulates apoptosis, autophagy, transcription, translation, and actin cytoskeletal reorganization. Its key roles include coordinating apoptotic and autophagy signaling, influencing smooth muscle contraction, and regulating starvation-induced autophagy. DAPK3/ZIPK Protein, Human (sf9, GST) is the recombinant human-derived DAPK3/ZIPK protein, expressed by Sf9 insect cells , with N-GST labeled tag.

DAPK3/ZIPK, a serine/threonine kinase, intricately regulates diverse cellular processes such as apoptosis, autophagy, transcription, translation, and actin cytoskeleton reorganization. It plays a crucial role in orchestrating both type I apoptotic and type II autophagic cell death signals, depending on the cellular context. DAPK3/ZIPK also governs smooth muscle contraction and is implicated in the regulation of starvation-induced autophagy. Its influence on myosin phosphorylation extends to both smooth muscle and non-muscle cells, where it directly phosphorylates MYL12B and MYL9 or inhibits smooth muscle myosin phosphatase (SMPP1M) via PPP1R12A phosphorylation. In non-muscle cells, it phosphorylates MYL12B, leading to actin cytoskeleton reorganization. Isoform 2, an alternative form of DAPK3/ZIPK, contributes to myosin, PPP1R12A, and MYL12B phosphorylation, inducing the condensation of actin stress fibers into thick bundles. Additionally, DAPK3/ZIPK plays a role in actin filament focal adhesion dynamics, and its function is modulated by RhoD. Moreover, it positively regulates canonical Wnt/beta-catenin signaling, interacts with NLK and TCF7L2, phosphorylates RPL13A upon interferon-gamma activation, enhancing its involvement in transcript-selective translation inhibition. DAPK3/ZIPK's diverse functions also extend to the regulation of cell cycle progression and cell proliferation, potentially acting as a tumor suppressor.

The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-GST

O43293-1 (M1-R454)

1613  [NCBI]

Full Length of Isoform-1

MSTFRQEDVEDHYEMGEELGSGQFAIVRKCRQKGTGKEYAAKFIKKRRLSSSRRGVSREEIEREVNILREIRHPNIITLHDIFENKTDVVLILELVSGGELFDFLAEKESLTEDEATQFLKQILDGVHYLHSKRIAHFDLKPENIMLLDKNVPNPRIKLIDFGIAHKIEAGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGETKQETLTNISAVNYDFDEEYFSNTSELAKDFIRRLLVKDPKRRMTIAQSLEHSWIKAIRRRNVRGEDSGRKPERRRLKTTRLKEYTIKSHSSLPPNNSYADFERFSKVLEEAAAAEEGLRELQRSRRLCHEDVEALAAIYEEKEAWYREESDSLGQDLRRLRQELLKTEALKRQAQEEAKGALLGTSGLKRRFSRLENRYEALAKQVASEMRFVQDLVRALEQEKLQGVECGLR

Approximately 70 kDa

≥ 85%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10 mM GSH, pH 7.4
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.