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Deoxyhypusine synthase/DHS Protein, Human (His)

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The deoxyhorseuridine synthase (DHS) protein plays a crucial role in the NAD-dependent oxidative cleavage of spermidine, followed by the transfer of the butylamine moiety of spermidine to the ε-amino group of the eIF-5A precursor protein. On the key lysine. Deoxyhypusine synthase/DHS Protein, Human (His) is the recombinant human-derived Deoxyhypusine synthase/DHS protein, expressed by E. coli , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Deoxyhypusine synthase/DHS Protein, Human (His)
  • Deoxyhypusine synthase/DHS Protein, Human (His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The deoxyhorseuridine synthase (DHS) protein plays a crucial role in the NAD-dependent oxidative cleavage of spermidine, followed by the transfer of the butylamine moiety of spermidine to the ε-amino group of the eIF-5A precursor protein. On the key lysine. Deoxyhypusine synthase/DHS Protein, Human (His) is the recombinant human-derived Deoxyhypusine synthase/DHS protein, expressed by E. coli , with C-6*His labeled tag.

Background

Deoxyhypusine synthase (DHS) plays a crucial role in the post-translational modification of the eIF-5A precursor protein. Catalyzing the NAD-dependent oxidative cleavage of spermidine, DHS subsequently transfers the butylamine moiety of spermidine to the epsilon-amino group of a lysine residue on the eIF-5A precursor. This process leads to the formation of the intermediate deoxyhypusine residue, representing the initial step in the conversion of the lysine into an atypical amino acid called hypusine. Hypusination is a unique modification specific to mature eIF-5A and is essential for its proper functioning.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag C-6*His
  • Accession

    P49366 (M1-D369)

  • Gene ID
    1725  [NCBI]
  • Molecular Construction
    • N-term
    • DHS (M1-D369)
      Accession # P49366
    • 6*His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    DHPS; DHS; Deoxyhypusine Synthase; DS; Migration-Inducing Gene 13; NEDSSWI; MIG13

  • AA Sequence

    MEGSLEREAPAGALAAVLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEPLSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVEEDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNTEGVKWTPSKMIARLGKEINNPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSYKNPGLVLDIVEDLRLINTQAIFAKCTGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLVAETFAQKMDAFMHEKNED

  • Predicted Molecular Mass

    42 kDa

  • Molecular Weight

    Approximately 42 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

1.Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 20% glycerol, pH 8.0.
2.Supplied as a 0.22 μm filtered solution of 20 mM Citrate, 8% trehalose, 0.03% Tween 20, pH 5.0.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Deoxyhypusine synthase/DHS Protein, Human (His) Product Image
    1/16
  • Experimental Validation Results for Deoxyhypusine synthase/DHS Protein, Human (His)
  • Deoxyhypusine synthase/DHS Protein, Human (His) Product Image