DLAT Protein, Human (sf9, His)

DLAT protein is an important component of the pyruvate dehydrogenase complex, which mainly promotes the conversion of pyruvate into acetyl-CoA and CO2. This enzymatic step establishes a critical link between glycolysis and the tricarboxylic acid (TCA) cycle. DLAT Protein, Human (sf9, His) is the recombinant human-derived DLAT protein, expressed by Sf9 insect cells , with N-His labeled tag.

DLAT protein, an essential component of the pyruvate dehydrogenase complex, plays a central role in the intricate process of converting pyruvate into acetyl-CoA and CO2. This enzymatic transformation is a critical step that establishes a pivotal connection between the glycolytic pathway and the tricarboxylic acid (TCA) cycle. DLAT's catalytic activity is instrumental in channeling pyruvate-derived metabolites into the TCA cycle, enabling the efficient utilization of carbon compounds for energy production and maintaining cellular metabolic equilibrium.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

P10515 (S87-L647)

1737  [NCBI]

Full Length of Mature Protein

SLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPTPPPVAAVPPTPQPLAPTPSAPCPATPAGPKGRVFVSPLAKKLAVEKGIDLTQVKGTGPDGRITKKDIDSFVPSKVAPAPAAVVPPTGPGMAPVPTGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSIDVNMGEVLLVRKELNKILEGRSKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVASMMSVTLSCDHRVVDGAVGAQWLAEFRKYLEKPITMLL

Approximately 75-77 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 85%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 0.2 mM PMSF.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping with dry ice.