1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. DLAT Protein, Human (sf9, His)

DLAT Protein, Human (sf9, His)

Cat. No.: HY-P75286
COA Handling Instructions

DLAT protein is an important component of the pyruvate dehydrogenase complex, which mainly promotes the conversion of pyruvate into acetyl-CoA and CO2. This enzymatic step establishes a critical link between glycolysis and the tricarboxylic acid (TCA) cycle. DLAT Protein, Human (sf9, His) is the recombinant human-derived DLAT protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of DLAT Protein, Human (sf9, His) is 561 a.a., with molecular weight of ~62.1 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

DLAT protein is an important component of the pyruvate dehydrogenase complex, which mainly promotes the conversion of pyruvate into acetyl-CoA and CO2. This enzymatic step establishes a critical link between glycolysis and the tricarboxylic acid (TCA) cycle. DLAT Protein, Human (sf9, His) is the recombinant human-derived DLAT protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of DLAT Protein, Human (sf9, His) is 561 a.a., with molecular weight of ~62.1 kDa.

Background

DLAT protein, an essential component of the pyruvate dehydrogenase complex, plays a central role in the intricate process of converting pyruvate into acetyl-CoA and CO2. This enzymatic transformation is a critical step that establishes a pivotal connection between the glycolytic pathway and the tricarboxylic acid (TCA) cycle. DLAT's catalytic activity is instrumental in channeling pyruvate-derived metabolites into the TCA cycle, enabling the efficient utilization of carbon compounds for energy production and maintaining cellular metabolic equilibrium.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

Sf9 insect cells

Tag

N-His

Accession

P10515 (S87-L647)

Gene ID
Molecular Construction
N-term
His
DLAT (S87-L647)
Accession # P10515
C-term
Synonyms
Pyruvate dehydrogenase complex component E2; PBC; PDC-E2; DLAT; DLTA
Molecular Weight

Approximately 62.1 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 200 mM NaCl, pH 7.5, 10 % glycerol, 0.5 mM TCEP.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

DLAT Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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DLAT Protein, Human (sf9, His)
Cat. No.:
HY-P75286
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