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DLK-1 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

DLK-1 Protein is implicated in neuroendocrine differentiation, signifying its role in cellular specialization. It acts as an inhibitor of adipocyte differentiation, regulating adipogenesis. Structurally, DLK-1 exists as a monomer, defining its molecular composition in these processes. Interactions with SH3RF2 underscore its engagement with cellular components and potential regulatory pathways. DLK-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived DLK-1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DLK-1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for DLK-1 Protein, Mouse (HEK293, His)
  • DLK-1 Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

DLK-1 Protein is implicated in neuroendocrine differentiation, signifying its role in cellular specialization. It acts as an inhibitor of adipocyte differentiation, regulating adipogenesis. Structurally, DLK-1 exists as a monomer, defining its molecular composition in these processes. Interactions with SH3RF2 underscore its engagement with cellular components and potential regulatory pathways. DLK-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived DLK-1 protein, expressed by HEK293 , with C-His labeled tag.

Background

The DLK-1 protein is implicated in potential roles related to neuroendocrine differentiation, indicating its involvement in the complex processes governing cellular specialization. Moreover, it functions as an inhibitor of adipocyte differentiation, suggesting a regulatory role in adipogenesis. Structurally, DLK-1 exists as a monomer, highlighting its singular molecular composition in these physiological processes. Additionally, the protein interacts with SH3RF2, emphasizing its engagement with other cellular components and potential regulatory pathways, as inferred by similarity.

Verified Bioactivity

Immobilized Mouse DLK-1 at 1 μg/mL (100 μL/well) can bind Anti-DLK1 Antibody. The ED50 for this effect is 266.5 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Mouse DLK-1 at 1 μg/mL (100 μL/well) can bind Anti-DLK1 Antibody, The ED50 for this effect is 266.5 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    Q09163-1 (A24-Q305)

  • Gene ID
    13386  [NCBI]
  • Molecular Construction
    • N-term
    • DLK-1 (A24-Q305)
      Accession # Q09163-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    DLK1; DLK; Delta Like Non-Canonical Notch Ligand 1; Delta-Like 1 Homolog (Drosophila); PG2; Delta-Like Homolog (Drosophila); Protein Delta Homolog 1; Preadipocyte Factor 1; Pref-1; Delta-Like 1 Homolog; Delta1; Fetal Antigen 1; FA1; Delta-Like 1; ZOG; Sec

  • AA Sequence

    AECDPPCDPQYGFCEADNVCRCHVGWEGPLCDKCVTAPGCVNGVCKEPWQCICKDGWDGKFCEIDVRACTSTPCANNGTCVDLEKGQYECSCTPGFSGKDCQHKAGPCVINGSPCQHGGACVDDEGQASHASCLCPPGFSGNFCEIVAATNSCTPNPCENDGVCTDIGGDFRCRCPAGFVDKTCSRPVSNCASGPCQNGGTCLQHTQVSFECLCKPPFMGPTCAKKRGASPVQVTHLPSGYGLTYRLTPGVHELPVQQPEQHILKVSMKELNKSTPLLTEGQ

  • Molecular Weight

    Approximately 50-68 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Mouse DLK-1 at 1 μg/mL (100 μL/well) can bind Anti-DLK1 Antibody, The ED50 for this effect is 266.5 ng/mL.

  • DLK-1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for DLK-1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for DLK-1 Protein, Mouse (HEK293, His)
  • DLK-1 Protein, Mouse (HEK293, His) Product Image