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DMP-1 Protein, Human (HEK293, His)

2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

DMP-1 protein exhibits dual functions in osteoblast differentiation. In undifferentiated osteoblast nuclei, its nonphosphorylated form activates osteoblast-specific genes. DMP-1 Protein, Human (HEK293, His) is the recombinant human-derived DMP-1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DMP-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for DMP-1 Protein, Human (HEK293, His)
  • DMP-1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

DMP-1 protein exhibits dual functions in osteoblast differentiation. In undifferentiated osteoblast nuclei, its nonphosphorylated form activates osteoblast-specific genes. DMP-1 Protein, Human (HEK293, His) is the recombinant human-derived DMP-1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

DMP-1 Protein appears to exhibit a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, its unphosphorylated form functions as a transcriptional component, activating osteoblast-specific genes such as osteocalcin. As osteoblasts transition to osteocytes, DMP-1 undergoes phosphorylation and is subsequently exported into the extracellular matrix. In this extracellular environment, it plays a regulatory role in the nucleation of hydroxyapatite, suggesting its involvement in the mineralization process. DMP-1's intricate functions underscore its significance in the molecular events that govern osteoblast differentiation and bone formation. Notably, DMP-1 interacts with importin alpha, adding a layer of complexity to its cellular dynamics and potential regulatory mechanisms.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha v beta 3 at 2 µg/mL can bind DMP-1. The ED50 for this effect is 6.245-6.798 nM.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha v beta 3 at 2 µg/mL can bind DMP-1 .The ED50 for this effect is 6.245 nM.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q13316 (L17-Y513)

  • Gene ID
    1758  [NCBI]
  • Molecular Construction
    • N-term
    • DMP-1 (L17-Y513)
      Accession # Q13316
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    DMP1; Dentin Matrix Acidic Phosphoprotein; Dentin Matrix Acidic Phosphoprotein 1; ARHP; Dentin Matrix Protein 1; ARHR; DMP-1

  • AA Sequence

    LPVTRYQNNESEDSEEWKGHLAQAPTPPLESSESSEGSKVSSEEQANEDPSDSTQSEEGLGSDDHQYIYRLAGGFSRSTGKGGDDKDDDEDDSGDDTFGDDDSGPGPKDRQEGGNSRLGSDEDSDDTIQASEESAPQGQDSAQDTTSESRELDNEDRVDSKPEGGDSTQESESEEHWVGGGSDGESSHGDGSELDDEGMQSDDPESIRSERGNSRMNSAGMKSKESGENSEQANTQDSGGSQLLEHPSRKIFRKSRISEEDDRSELDDNNTMEEVKSDSTENSNSRDTGLSQPRRDSKGDSQEDSKENLSQEESQNVDGPSSESSQEANLSSQENSSESQEEVVSESRGDNPDPTTSYVEDQEDSDSSEEDSSHTLSHSKSESREEQADSESSESLNFSEESPESPEDENSSSQEGLQSHSSSAESQSEESHSEEDDSDSQDSSRSKEDSNSTESKSSSEEDGQLKNIEIESRKLTVDAYHNKPIGDQDDNDCQDGY

  • Molecular Weight

    Approximately 45-120 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM His-HCl, 6% trehalose, 4% mannitol, 0.05% Tween 80, pH 6.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha v beta 3 at 2 µg/mL can bind DMP-1 .The ED50 for this effect is 6.245 nM.

  • DMP-1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for DMP-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for DMP-1 Protein, Human (HEK293, His)
  • DMP-1 Protein, Human (HEK293, His) Product Image