DPAGT1 Protein, Human (sf9)
DPAGT1 Protein, Human (sf9) is the recombinant human-derived DPAGT1, expressed by sf9 insect cells , with tag Free labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
DPAGT1 Protein, Human (sf9) is the recombinant human-derived DPAGT1, expressed by sf9 insect cells , with tag Free labeled tag.
Background
DPAGT1 is a UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that functions in the biosynthetic pathway of dolichol-linked oligosaccharides, which serve as glycan precursors for protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides initiates on the cytosolic side of the endoplasmic reticulum membrane and concludes in its lumen. Sequential sugar additions to dolichol pyrophosphate produce dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses, and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. DPAGT1 catalyzes the first step of dolichol-linked oligosaccharide biosynthesis, transferring GlcNAc-1-P from cytosolic UDP-GlcNAc to the carrier lipid dolichyl phosphate (P-dolichol), resulting in GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag Tag Free
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Accession
Q9H3H5-1 (M1-V408)
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Gene ID1798
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Protein Length
Full Length of Isoform-1
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Synonyms
DPAGT1; UDP-N-Acetylglucosamine--Dolichyl-Phosphate N-Acetylglucosaminephosphotransferase 1; Prev. DPAGT2; GlcNAc-1-P Transferase; Prev. DPAGT; G1PT; GPT; Dolichyl-Phosphate (UDP-N-Acetylglucosamine) N-Acetylglucosaminephosphotransferase 1 (GlcNAc-1-P Tra
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AA Sequence
MWAFSELPMPLLINLIVSLLGFVATVTLIPAFRGHFIAARLCGQDLNKTSRQQIPESQGVISGAVFLIILFCFIPFPFLNCFVKEQCKAFPHHEFVALIGALLAICCMIFLGFADDVLNLRWRHKLLLPTAASLPLLMVYFTNFGNTTIVVPKPFRPILGLHLDLGILYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVFNLVELEGDCRDDHVFSLYFMIPFFFTTLGLLYHNWYPSRVFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFMPQVFNFLYSLPQLLHIIPCPRHRIPRLNIKTGKLEMSYSKFKTKSLSFLGTFILKVAESLQLVTVHQSETEDGEFTECNNMTLINLLLKVLGPIHERNLTLLLLLLQILGSAITFSIRYQLVRLFYDV
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Predicted Molecular Mass
46.2 kDa
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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