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DSC2/Desmocollin-2 Protein, Human (HEK293, C-His)

Customer Review

Based on 1 Customer Validation

DSC2/Desmocollin-2 protein is vital for intercellular desmosome junctions, mediating adhesion by interacting with plaque proteins and intermediate filaments. It also regulates cell positioning and interacts with DSP, PKP2, and JUP, strengthening junction stability. Overall, DSC2/Desmocollin-2 protein maintains tissue organization and functionality. DSC2/Desmocollin-2 Protein, Human (HEK293, C-His) is the recombinant human-derived DSC2/Desmocollin-2, expressed by HEK293, with C-10*His labeled tag. The total length of DSC2/Desmocollin-2 Protein, Human (HEK293, C-His) is 657 a.a..

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DSC2/Desmocollin-2 Protein, Human (HEK293, C-His)
  • Experimental Validation Results for DSC2/Desmocollin-2 Protein, Human (HEK293, C-His)
  • DSC2/Desmocollin-2 Protein, Human (HEK293, C-His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

DSC2/Desmocollin-2 protein is vital for intercellular desmosome junctions, mediating adhesion by interacting with plaque proteins and intermediate filaments. It also regulates cell positioning and interacts with DSP, PKP2, and JUP, strengthening junction stability. Overall, DSC2/Desmocollin-2 protein maintains tissue organization and functionality. DSC2/Desmocollin-2 Protein, Human (HEK293, C-His) is the recombinant human-derived DSC2/Desmocollin-2, expressed by HEK293, with C-10*His labeled tag. The total length of DSC2/Desmocollin-2 Protein, Human (HEK293, C-His) is 657 a.a..

Background

DSC2/Desmocollin-2 protein is a critical component of intercellular desmosome junctions, playing a crucial role in facilitating cell-cell adhesion by mediating the interaction between plaque proteins and intermediate filaments. In addition to its adhesive function, DSC2 may also contribute to epidermal cell positioning by regulating the adhesiveness between cells expressing different isoforms. This protein interacts with DSP, PKP2, and JUP, further reinforcing the stability and integrity of these junctions. By participating in these molecular interactions, DSC2/Desmocollin-2 protein ensures the proper organization and functionality of tissues.

Verified Bioactivity

Measured by its ability to inhibit the proliferation of PC-3 cells. The ED50 for this effect is 0.8445 μg/mL, corresponding to a specific activity is 1184.13 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit the proliferation of PC-3 cells. The ED50 for this effect is 0.8445 μg/mL, corresponding to a specific activity is 1184.13 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q02487 (A28-R684)

  • Gene ID
    1824
  • Protein Length

    Partial Extracellular Domain (with Propeptide)

  • Synonyms

    DSC2; Desmosomal Glycoprotein III; Prev. DSC3; Desmocollin-3; CDHF2; DGII/III; Cadherin Family Member 2; ARVD11; Desmocollin-2; DG2; Desmosomal Glycoprotein II/III; Desmocollin 2; Desmosomal Glycoprotein II

  • AA Sequence

    ASDACKNVTLHVPSKLDAEKLVGRVNLKECFTAANLIHSSDPDFQILEDGSVYTTNTILLSSEKRSFTILLSNTENQEKKKIFVFLEHQTKVLKKRHTKEKVLRRAKRRWAPIPCSMLENSLGPFPLFLQQVQSDTAQNYTIYYSIRGPGVDQEPRNLFYVERDTGNLYCTRPVDREQYESFEIIAFATTPDGYTPELPLPLIIKIEDENDNYPIFTEETYTFTIFENCRVGTTVGQVCATDKDEPDTMHTRLKYSIIGQVPPSPTLFSMHPTTGVITTTSSQLDRELIDKYQLKIKVQDMDGQYFGLQTTSTCIINIDDVNDHLPTFTRTSYVTSVEENTVDVEILRVTVEDKDLVNTANWRANYTILKGNENGNFKIVTDAKTNEGVLCVVKPLNYEEKQQMILQIGVVNEAPFSREASPRSAMSTATVTVNVEDQDEGPECNPPIQTVRMKENAEVGTTSNGYKAYDPETRSSSGIRYKKLTDPTGWVTIDENTGSIKVFRSLDREAETIKNGIYNITVLASDQGGRTCTGTLGIILQDVNDNSPFIPKKTVIICKPTMSSAEIVAVDPDEPIHGPPFDFSLESSTSEVQRMWRLKAINDTAARLSYQNDPPFGSYVVPITVRDRLGMSSVTSLDVTLCDCITENDCTHRVDPR

  • Predicted Molecular Mass

    73.8 kDa

  • Molecular Weight

    Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit the proliferation of PC-3 cells. The ED50 for this effect is 0.8445 μg/mL, corresponding to a specific activity is 1184.13 units/mg.

  • DSC2/Desmocollin-2 Protein, Human (HEK293, C-His) Product Image
    1/16
  • Experimental Validation Results for DSC2/Desmocollin-2 Protein, Human (HEK293, C-His)
  • Experimental Validation Results for DSC2/Desmocollin-2 Protein, Human (HEK293, C-His)
  • DSC2/Desmocollin-2 Protein, Human (HEK293, C-His) Product Image