DSC2/Desmocollin-2 Protein, Rat (HEK293, His)

DSC2 (or Desmocollin-2) is an important component of desmosome connections between cells and plays a key role in the complex interaction network between plaque proteins and intermediate filaments. At these junctions, DSC2 contributes to cell-cell adhesion, promoting tissue cohesion and stability. DSC2/Desmocollin-2 Protein, Rat (HEK293, His) is the recombinant rat-derived DSC2/Desmocollin-2 protein, expressed by HEK293 , with C-His labeled tag. The total length of DSC2/Desmocollin-2 Protein, Rat (HEK293, His) is 666 a.a., with molecular weight of ~80-95 KDa, respectively.

Desmocollin-2 (DSC2) is a crucial component of intercellular desmosome junctions, playing a pivotal role in cell-cell adhesion. As part of desmosomes, DSC2 contributes to the intricate network that mediates cell-cell interactions. These junctions are essential for the maintenance of tissue integrity and provide structural support through the interaction of plaque proteins and intermediate filaments. DSC2's involvement in this molecular complex underscores its significance in ensuring the cohesion and stability of tissues, particularly those subjected to mechanical stress. The protein's function in promoting cell-cell adhesion highlights its importance in the maintenance of tissue architecture and overall cellular integrity. (

Measured by the ability of the immobilized protein to support the adhesion of the Caki-2 human clear cell carcinoma epithelial cells. The adhesion rate for this effect is 75.84% in the presence of 10 μg/mL Rat DSC2.

Rat

HEK293

C-His

Q3T1K6 (C29-P694)

291760  [NCBI]

CDACKEVVLQVPSELPAGKFVGRVNLMECLKSANVIHLSDPDFQVFEDGSVYTTNSVVLSSGQRSFTIWLFNTDSQEERELSVHLEGPVEVLNQRLHTEKVLRRAKRRWAPIPCSMMENSLGPFPLFLQQIQSDKAQNYTIYYSISGPGVDKEPVGLFRIDRDTGNLYCTDRVDREQYDSFELTALAMTPDGYMVENLLTLLIKVEDENDNYPIFTQKVYSFTVLENSPFGSHVGEVCATDKDEPDTMHTRLKYSILEQSPSPPMLFTMHPRTGVITTTSTQLDRELIDKYQLIIKVQDMDGQYFGLQTTATCVITIGDVNDNLPTFTRTTYVTSVEENTVNVELLRLSVQDKDLVNSPNWRANYTILKGNENGNFKIKTDPQTNEGILCVIMPLDYEERQRVTLEIGVVNEAPYTRQAGSKSSMSTATVTVNVKNQDEGPECIPQTQTVRIRENVPIGTRNDGYKAYDPETKNSIGIRYRKLSDPKGWITVNEDSGSITIFRTLDREAETIRNGIYNITVLALDADGRSCTGTLGILLEDVNDNGPFIPKQTVVICRATMSSAEIVAVDPDEPANGPPFDFSLESSDSEVRRRWKLTRINDTAASLSYQNDPAFDSYVIPVRVTDRFGLSSVTSLNVVVCDCITESDCTYRTGERTGYADVRLGP

Approximately 80-95 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.