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DSPG3/Epiphycan Protein, Human (HEK293, His)

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Based on 1 Customer Validation

DSPG3/Epiphycan Protein plays a crucial role in bone formation and the establishment of ordered cartilage structure by contributing to matrix organization. Its multifaceted function in skeletal development influences dynamic processes related to bone formation and cartilage structural integrity. The protein's dual impact underscores its significance in maintaining the integrity and organization of the extracellular matrix in skeletal tissues. DSPG3/Epiphycan Protein, Human (HEK293, His) is the recombinant human-derived DSPG3/Epiphycan protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DSPG3/Epiphycan Protein, Human (HEK293, His)
  • Experimental Validation Results for DSPG3/Epiphycan Protein, Human (HEK293, His)
  • DSPG3/Epiphycan Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

DSPG3/Epiphycan Protein plays a crucial role in bone formation and the establishment of ordered cartilage structure by contributing to matrix organization. Its multifaceted function in skeletal development influences dynamic processes related to bone formation and cartilage structural integrity. The protein's dual impact underscores its significance in maintaining the integrity and organization of the extracellular matrix in skeletal tissues. DSPG3/Epiphycan Protein, Human (HEK293, His) is the recombinant human-derived DSPG3/Epiphycan protein, expressed by HEK293 , with C-His labeled tag.

Background

The DSPG3/Epiphycan protein appears to play a crucial role in both bone formation and the establishment of the ordered structure of cartilage by contributing to matrix organization. Its involvement suggests a multifaceted function in skeletal development, potentially influencing the dynamic processes associated with bone formation and the structural integrity of cartilage. The protein's dual impact on these essential aspects underscores its significance in maintaining the integrity and organization of the extracellular matrix in skeletal tissues.

Verified Bioactivity

Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. The ED50 for this effect is 0.1403 μg/mL in the presence of 0.5 μg/mL Fibronectin, corresponding to a specific activity is 7.127×10^3 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. The ED50 for this effect is 0.1403 μg/mL in the presence of 0.5 μg/mL Fibronectin, corresponding to a specific activity is 7.127×10^3 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q99645 (A20-V322)

  • Gene ID
    1833  [NCBI]
  • Molecular Construction
    • N-term
    • DSPG3 (A20-V322)
      Accession # Q99645
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    EPYC; Small Chondroitin/Dermatan Sulfate Proteoglycan; Prev. DSPG3; Proteoglycan-Lb; SLRR3B; PGLB; Dermatan Sulfate Proteoglycan 3; Dermatan Sulphate Proteoglycan 3; Pg-Lb; PG-Lb; Epiphycan; Epiphycan Proteoglycan

  • AA Sequence

    APTLESINYDSETYDATLEDLDNLYNYENIPVDKVEIEIATVMPSGNRELLTPPPQPEKAQEEEEEEESTPRLIDGSSPQEPEFTGVLGPHTNEDFPTCLLCTCISTTVYCDDHELDAIPPLPKNTAYFYSRFNRIKKINKNDFASLSDLKRIDLTSNLISEIDEDAFRKLPQLRELVLRDNKIRQLPELPTTLTFIDISNNRLGRKGIKQEAFKDMYDLHHLYLTDNNLDHIPLPLPENLRALHLQNNNILEMHEDTFCNVKNLTYIRKALEDIRLDGNPINLSKTPQAYMCLPRLPVGSLV

  • Molecular Weight

    Approximately 43-52 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. The ED50 for this effect is 0.1403 μg/mL in the presence of 0.5 μg/mL Fibronectin, corresponding to a specific activity is 7.127×10^3 units/mg.

  • DSPG3/Epiphycan Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for DSPG3/Epiphycan Protein, Human (HEK293, His)
  • Experimental Validation Results for DSPG3/Epiphycan Protein, Human (HEK293, His)
  • DSPG3/Epiphycan Protein, Human (HEK293, His) Product Image