DSPG3/Epiphycan Protein, Human (HEK293, His)

DSPG3/Epiphycan Protein plays a crucial role in bone formation and the establishment of ordered cartilage structure by contributing to matrix organization. Its multifaceted function in skeletal development influences dynamic processes related to bone formation and cartilage structural integrity. The protein's dual impact underscores its significance in maintaining the integrity and organization of the extracellular matrix in skeletal tissues. DSPG3/Epiphycan Protein, Human (HEK293, His) is the recombinant human-derived DSPG3/Epiphycan protein, expressed by HEK293 , with C-His labeled tag.

The DSPG3/Epiphycan protein appears to play a crucial role in both bone formation and the establishment of the ordered structure of cartilage by contributing to matrix organization. Its involvement suggests a multifaceted function in skeletal development, potentially influencing the dynamic processes associated with bone formation and the structural integrity of cartilage. The protein's dual impact on these essential aspects underscores its significance in maintaining the integrity and organization of the extracellular matrix in skeletal tissues.

Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. The ED₅₀ for this effect is 0.1403 μg/mL in the presence of 0.5 μg/mL Fibronectin, corresponding to a specific activity is 7.127×10^3 units/mg.

• 
  Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. The ED₅₀ for this effect is 0.1403 μg/mL in the presence of 0.5 μg/mL Fibronectin, corresponding to a specific activity is 7.127×10^3 units/mg.

Human

HEK293

C-His

Q99645 (A20-V322)

1833  [NCBI]

Full Length of Mature Protein

APTLESINYDSETYDATLEDLDNLYNYENIPVDKVEIEIATVMPSGNRELLTPPPQPEKAQEEEEEEESTPRLIDGSSPQEPEFTGVLGPHTNEDFPTCLLCTCISTTVYCDDHELDAIPPLPKNTAYFYSRFNRIKKINKNDFASLSDLKRIDLTSNLISEIDEDAFRKLPQLRELVLRDNKIRQLPELPTTLTFIDISNNRLGRKGIKQEAFKDMYDLHHLYLTDNNLDHIPLPLPENLRALHLQNNNILEMHEDTFCNVKNLTYIRKALEDIRLDGNPINLSKTPQAYMCLPRLPVGSLV

Approximately 43-52 kDa.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.