EAAT3 Protein, Human (HEK293, His, Strep, GFP)
EAAT3 Protein, Human (HEK293, His, Strep, GFP) is the recombinant human-derived EAAT3, expressed by HEK293 , with Strep, His, GFP labeled tag. ,
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
EAAT3 Protein, Human (HEK293, His, Strep, GFP) is the recombinant human-derived EAAT3, expressed by HEK293 , with Strep, His, GFP labeled tag. ,
Background
EAAT3 is a sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate, L-aspartate, and D-aspartate, with additional capability to transport L-cysteine. It functions as a symporter, co-transporting one amino acid molecule with two or three Na(+) ions and one proton while counter-transporting one K(+) ion. The protein also facilitates Cl(-) flux uncoupled from amino acid transport to prevent negative charge accumulation during aspartate/Na(+) symport. It plays a key role in renal reabsorption of L-glutamate and L-aspartate. By similar functions: it contributes redundantly to synaptic glutamate clearance for neurotransmission termination, participates in glutathione biosynthesis and oxidative stress protection via L-glutamate/L-cysteine transport, and is negatively regulated by ARL6IP5.
Technical Parameters
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Species Human
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Source HEK293
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Tag Strep;His;GFP
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Accession
P43005 (M1-F524, N178T, N195T)
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Gene ID6505
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Protein Length
Full Length
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Synonyms
SLC1A1; Sodium-Dependent Glutamate/Aspartate Transporter 3; Solute Carrier Family 1 Member 1; Neuronal And Epithelial Glutamate Transporter; HEAAC1; Excitatory Amino Acid Carrier 1; EAAC1; Excitatory Amino-Acid Carrier 1; EAAT3; Amino Acid Transporter; Ex
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AA Sequence
MGKPARKGCEWKRFLKNNWVLLSTVAAVVLGITTGVLVREHSNLSTLEKFYFAFPGEILMRMLKLIILPLIISSMITGVAALDSNVSGKIGLRAVVYYFCTTLIAVILGIVLVVSIKPGVTQKVGEIARTGSTPEVSTVDAMLDLIRNMFPENLVQACFQQYKTKREEVKPPSDPEMTMTEESFTAVMTTAISKTKTKEYKIVGMYSDGINVLGLIVFCLVFGLVIGKMGEKGQILVDFFNALSDATMKIVQIIMCYMPLGILFLIAGKIIEVEDWEIFRKLGLYMATVLTGLAIHSIVILPLIYFIVVRKNPFRFAMGMAQALLTALMISSSSATLPVTFRCAEENNQVDKRITRFVLPVGATINMDGTALYEAVAAVFIAQLNDLDLGIGQIITISITATSASIGAAGVPQAGLVTMVIVLSAVGLPAEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF
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Predicted Molecular Mass
87.1 kDa
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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