EAAT3 Protein, Human (HEK293, His, Strep, GFP)

EAAT3 Protein, Human (HEK293, His, Strep, GFP) is the recombinant human-derived EAAT3, expressed by HEK293 , with Strep, His, GFP labeled tag. ,

EAAT3 is a sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate, L-aspartate, and D-aspartate, with additional capability to transport L-cysteine. It functions as a symporter, co-transporting one amino acid molecule with two or three Na(+) ions and one proton while counter-transporting one K(+) ion. The protein also facilitates Cl(-) flux uncoupled from amino acid transport to prevent negative charge accumulation during aspartate/Na(+) symport. It plays a key role in renal reabsorption of L-glutamate and L-aspartate. By similar functions: it contributes redundantly to synaptic glutamate clearance for neurotransmission termination, participates in glutathione biosynthesis and oxidative stress protection via L-glutamate/L-cysteine transport, and is negatively regulated by ARL6IP5.

Human

HEK293

Strep;His;GFP

P43005 (M1-F524, N178T, N195T)

6505

Full Length

MGKPARKGCEWKRFLKNNWVLLSTVAAVVLGITTGVLVREHSNLSTLEKFYFAFPGEILMRMLKLIILPLIISSMITGVAALDSNVSGKIGLRAVVYYFCTTLIAVILGIVLVVSIKPGVTQKVGEIARTGSTPEVSTVDAMLDLIRNMFPENLVQACFQQYKTKREEVKPPSDPEMTMTEESFTAVMTTAISKTKTKEYKIVGMYSDGINVLGLIVFCLVFGLVIGKMGEKGQILVDFFNALSDATMKIVQIIMCYMPLGILFLIAGKIIEVEDWEIFRKLGLYMATVLTGLAIHSIVILPLIYFIVVRKNPFRFAMGMAQALLTALMISSSSATLPVTFRCAEENNQVDKRITRFVLPVGATINMDGTALYEAVAAVFIAQLNDLDLGIGQIITISITATSASIGAAGVPQAGLVTMVIVLSAVGLPAEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.