ECD Protein, Human (sf9, His-GST)

ECD, a versatile regulator in cellular dynamics, assumes a pivotal role in stabilizing and modulating the activity of the tumor suppressor p53/TP53. By inhibiting MDM2-mediated degradation of p53, ECD collaborates, potentially with TXNIP, to safeguard the integrity of this critical guardian of genomic stability. Beyond its role in p53 stabilization, ECD reveals a multifaceted nature, engaging in intricate transcriptional regulation. In vitro, it demonstrates intrinsic transactivation activity, further potentiated by its interaction with EP300, and may function as a transcriptional activator essential for the expression of glycolytic genes. Implicated in cell cycle progression, ECD is proposed to disrupt Rb-E2F binding, thereby activating E2F proteins, and this cell cycle-regulating function is intricately linked to its association with the RUVBL1-containing R2TP complex. Additionally, ECD's involvement in pre-mRNA splicing adds another layer to its regulatory repertoire. Interacting with a network of key proteins, including TP53, MDM2, TXNIP, PIH1D1, RUVBL1, RB1, RBL1, RBL2, and EP300, ECD emerges as a central player orchestrating diverse cellular processes.