EIF4E1 Protein, Human

EIF4E1 Protein, Human is the recombinant human-derived EIF4E1, expressed by E. coli , with tag Free labeled tag.

EIF4E1 is a component of the protein complex eIF4F, which is involved in recognizing the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structures, and recruiting mRNA to the ribosome. It recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step of protein synthesis and facilitates ribosome binding by inducing the unwinding of mRNA secondary structures (By similarity). Additionally, EIF4E1 is a key component of recessive resistance to potyviruses.

Human

E. coli

Tag Free

O23252 (S59-A235)

827529

Partial

SHPLEHSWTFWFDNPAVKSKQTSWGSSLRPVFTFSTVEEFWSLYNNMKHPSKLAHGADFYCFKHIIEPKWEDPICANGGKWTMTFPKEKSDKSWLYTLLALIGEQFDHGDEICGAVVNIRGKQERISIWTKNASNEAAQVSIGKQWKEFLDYNNSIGFIIHEDAKKLDRNAKNAYTA

Approximately 20 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200mM NaCl, 20% glycerol, 1mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.