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Endosialin/CD248 Protein, Mouse (HEK293, His)

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Endosialin/CD248 Protein potentially plays a role in angiogenesis or vascular function, suggesting involvement in blood vessel formation or vascular regulation. The exact mechanisms and contexts of Endosialin/CD248 in these processes are areas of interest, emphasizing its potential significance in vascular development and function. Endosialin/CD248 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Endosialin/CD248 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Endosialin/CD248 Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

Endosialin/CD248 Protein potentially plays a role in angiogenesis or vascular function, suggesting involvement in blood vessel formation or vascular regulation. The exact mechanisms and contexts of Endosialin/CD248 in these processes are areas of interest, emphasizing its potential significance in vascular development and function. Endosialin/CD248 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Endosialin/CD248 protein, expressed by HEK293 , with C-His labeled tag.

Background

The Endosialin/CD248 protein is suggested to potentially play a role in angiogenesis or vascular function, indicating its potential involvement in the intricate processes of blood vessel formation or vascular regulation. The exact mechanisms and specific contexts in which Endosialin/CD248 operates in these processes remain areas of interest, highlighting its potential significance in vascular development and function.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-8*His
  • Accession

    Q91V98 (Q18-L695)

  • Gene ID
    70445  [NCBI]
  • Molecular Construction
    • N-term
    • Endosialin (Q18-L695)
      Accession # Q91V98
    • 8*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CD248; CD248 Molecule, Endosialin; Prev. CD164L1; CD164 Sialomucin-Like 1; Tumor Endothelial Marker 1; 2610111G01Rik; Endosialin; CD248 Antigen; TEM1; CD248 Molecule; CD248 Antigen, Endosialin

  • AA Sequence

    QVPWTPEPRAACGPSSCYALFPRRRTFLEAWRACRELGGNLATPRTPEEAQRVDSLVGVGPANGLLWIGLQRQARQCQPQRPLRGFIWTTGDQDTAFTNWAQPATEGPCPAQRCAALEASGEHRWLEGSCTLAVDGYLCQFGFEGACPALPLEVGQAGPAVYTTPFNLVSSEFEWLPFGSVAAVQCQAGRGASLLCVKQPSGGVGWSQTGPLCPGTGCGPDNGGCEHECVEEVDGAVSCRCSEGFRLAADGHSCEDPCAQAPCEQQCEPGGPQGYSCHCRLGFRPAEDDPHRCVDTDECQIAGVCQQMCVNYVGGFECYCSEGHELEADGISCSPAGAMGAQASQDLRDELLDDGEEGEDEEEPWEDFDGTWTEEQGILWLAPTHPPDFGLPYRPNFPQDGEPQRLHLEPTWPPPLSAPRGPYHSSVVSATRPMVISATRPTLPSAHKTSVISATRPPLSPVHPPAMAPATPPAVFSEHQIPKIKANYPDLPFGHKPGITSATHPARSPPYQPPIISTNYPQVFPPHQAPMSPDTHTITYLPPVPPHLDPGDTTSKAHQHPLLPDAPGIRTQAPQLSVSALQPPLPTNSRSSVHETPVPAANQPPAFPSSPLPPQRPTNQTSSISPTHSYSRAPLVPREGVPSPKSVPQLPSVPSTAAPTALAESGLAGQSQRDDRWL

  • Predicted Molecular Mass

    73.6 kDa

  • Molecular Weight

    Approximately 75-140 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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