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Endothelin-1/EDN1 Protein, Human (GST)

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Based on 1 publication(s) in Google Scholar

Endothelin-1/EDN1, an endothelium-derived vasoconstrictor peptide, acts as a likely ligand for G-protein coupled receptors EDNRA and EDNRB. Its binding initiates a signaling cascade in glomerular mesangial cells involving PTK2B, BCAR1, BCAR3, and GTPases RAP1 and RHOA. Furthermore, Endothelin-1/EDN1 shows binding affinity for the DEAR/FBXW7-AS1 receptor, underscoring its involvement in diverse cellular signaling and vascular regulation. Endothelin-1/EDN1 Protein, Human (GST) is the recombinant human-derived Endothelin-1/EDN1 protein, expressed by E. coli , with N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Endothelin-1/EDN1 Protein, Human (GST)
  • Experimental Validation Results for Endothelin-1/EDN1 Protein, Human (GST)
  • Endothelin-1/EDN1 Protein, Human (GST) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

Endothelin-1/EDN1, an endothelium-derived vasoconstrictor peptide, acts as a likely ligand for G-protein coupled receptors EDNRA and EDNRB. Its binding initiates a signaling cascade in glomerular mesangial cells involving PTK2B, BCAR1, BCAR3, and GTPases RAP1 and RHOA. Furthermore, Endothelin-1/EDN1 shows binding affinity for the DEAR/FBXW7-AS1 receptor, underscoring its involvement in diverse cellular signaling and vascular regulation. Endothelin-1/EDN1 Protein, Human (GST) is the recombinant human-derived Endothelin-1/EDN1 protein, expressed by E. coli , with N-GST labeled tag.

Background

Endothelin-1/EDN1, a vasoconstrictor peptide derived from the endothelium, serves as a probable ligand for G-protein coupled receptors EDNRA and EDNRB. Upon binding, it activates a signaling cascade involving PTK2B, BCAR1, BCAR3, and GTPases RAP1 and RHOA in glomerular mesangial cells. Additionally, Endothelin-1/EDN1 exhibits binding affinity for the DEAR/FBXW7-AS1 receptor, contributing to its diverse roles in cellular signaling and vascular regulation.

Verified Bioactivity

1.Measured by its binding ability in a functional ELISA. Immobilized EDNRB at 5 μg/mL can bind human EDN1 with a linear range of 20-200 μg/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized EDNRB at 0.5 μg/mL can bind human EDN1 with a linear range of 200-250 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human EDNRB at 5 μg/mL (100 μL/well) on the plate can bind Biotinylated Human EDN1 Protein. The ED50 for this effect is 20.79 μg/mL.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-GST
  • Accession

    P05305 (C53-W73)

  • Gene ID
    1906  [NCBI]
  • Molecular Construction
    • N-term
    • GST
    • EDN1 (C53-W73)
      Accession # P05305
    • C-term
  • Protein Length

    Full Length of EDN1 Peptide

  • Synonyms

    EDN1; PPET1; Endothelin 1; ARCND3; Endothelin-1; HDLCQ7; ET1; QME; Preproendothelin-1

  • AA Sequence

    CSCSSLMDKECVYFCHLDIIW

  • Molecular Weight

    Approximately 28 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human EDNRB at 5 μg/mL (100 μL/well) on the plate can bind Biotinylated Human EDN1 Protein. The ED50 for this effect is 20.79 μg/mL.

  • Endothelin-1/EDN1 Protein, Human (GST) Product Image
    1/16
  • Experimental Validation Results for Endothelin-1/EDN1 Protein, Human (GST)
  • Experimental Validation Results for Endothelin-1/EDN1 Protein, Human (GST)
  • Endothelin-1/EDN1 Protein, Human (GST) Product Image