Endothelin-1/EDN1 Protein, Human (GST)

Endothelin-1/EDN1, an endothelium-derived vasoconstrictor peptide, acts as a likely ligand for G-protein coupled receptors EDNRA and EDNRB. Its binding initiates a signaling cascade in glomerular mesangial cells involving PTK2B, BCAR1, BCAR3, and GTPases RAP1 and RHOA. Furthermore, Endothelin-1/EDN1 shows binding affinity for the DEAR/FBXW7-AS1 receptor, underscoring its involvement in diverse cellular signaling and vascular regulation. Endothelin-1/EDN1 Protein, Human (GST) is the recombinant human-derived Endothelin-1/EDN1 protein, expressed by E. coli , with N-GST labeled tag.

Endothelin-1/EDN1, a vasoconstrictor peptide derived from the endothelium, serves as a probable ligand for G-protein coupled receptors EDNRA and EDNRB. Upon binding, it activates a signaling cascade involving PTK2B, BCAR1, BCAR3, and GTPases RAP1 and RHOA in glomerular mesangial cells. Additionally, Endothelin-1/EDN1 exhibits binding affinity for the DEAR/FBXW7-AS1 receptor, contributing to its diverse roles in cellular signaling and vascular regulation.

1.Measured by its binding ability in a functional ELISA. Immobilized EDNRB at 5 μg/mL can bind human EDN1 with a linear range of 20-200 μg/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized EDNRB at 0.5 μg/mL can bind human EDN1 with a linear range of 200-250 μg/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Human EDNRB at 5 μg/mL (100 μL/well) on the plate can bind Biotinylated Human EDN1 Protein. The ED₅₀ for this effect is 20.79 μg/mL.

Human

E. coli

N-GST

P05305 (C53-W73)

1906  [NCBI]

Full Length of EDN1

CSCSSLMDKECVYFCHLDIIW

Approximately 28 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.