Ephrin-B2/EFNB2 Protein, Rat (HEK293, His)

Ephrin-B2 (EFNB2) is a member of the ephrin family. Ephrin-B2/EFNB2 Protein, Rat (HEK293, His) is the recombinant rat-derived Ephrin-B2/EFNB2 protein, expressed by HEK293 , with C-His, C-6*His labeled tag.

Ephrin-B2, also known as EFNB2, is a protein that plays a crucial role in cellular communication and tissue development. Belonging to the ephrin family, Ephrin-B2 is a transmembrane protein that acts as both a ligand and a receptor, mediating bidirectional signaling events. Its primary function is to interact with Eph receptors on neighboring cells, initiating a cascade of intracellular signals that regulate various cellular processes, including cell adhesion, migration, and tissue patterning. Ephrin-B2 is particularly implicated in angiogenesis, the formation of new blood vessels, where it governs endothelial cell behavior and influences vascular development. Additionally, it has been associated with diverse physiological and pathological processes, such as neuronal guidance, immune response, and cancer progression. Understanding the intricate functions of Ephrin-B2 is crucial for unraveling its therapeutic potential in various medical contexts.

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat Ephrin B2 Protein at 10 μg/mL (100 μL/well) can bind Mouse EphB4 Protein. The ED₅₀ for this effect is 28.91 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat Ephrin B2 Protein at 10 μg/mL (100 μL/well) can bind Mouse EphB4 Protein. The ED₅₀ for this effect is 28.91 ng/mL.

Rat

HEK293

C-6*His

B2B9A9/NP_001100798.1 (R29-A229)

306636  [NCBI]

Partial

RSIVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKEQADRCTIKKENTPLLNCARPDQDVKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSTRNNDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNLLGSEVA

33-39 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.