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ERG Protein, Human (P.pastoris, His)

Cat. No.: HY-P71733
COA Handling Instructions

ERG Protein, a transcriptional regulator, recruits SETDB1 histone methyltransferase to modify local chromatin. It interacts with SETDB1 and is identified in an IGF2BP1-dependent mRNP granule complex with untranslated mRNAs. ERG Protein, Human (P.pastoris, His) is the recombinant human-derived ERG protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of ERG Protein, Human (P.pastoris, His) is 486 a.a., with molecular weight of ~57 kDa.

For research use only. We do not sell to patients.

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Description

ERG Protein, a transcriptional regulator, recruits SETDB1 histone methyltransferase to modify local chromatin. It interacts with SETDB1 and is identified in an IGF2BP1-dependent mRNP granule complex with untranslated mRNAs. ERG Protein, Human (P.pastoris, His) is the recombinant human-derived ERG protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of ERG Protein, Human (P.pastoris, His) is 486 a.a., with molecular weight of ~57 kDa.

Background

ERG, a transcriptional regulator, is implicated in the modulation of gene expression by potentially facilitating transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. Moreover, ERG has been identified as part of an IGF2BP1-dependent mRNP granule complex that contains untranslated mRNAs, suggesting its involvement in post-transcriptional processes. The interaction with SETDB1 underscores its role in the dynamic interplay of molecular components within cellular pathways, contributing to the intricate regulation of gene expression and chromatin organization.

Species

Human

Source

P. pastoris

Tag

N-6*His

Accession

P11308-3 (M1-Y486)

Gene ID
Molecular Construction
N-term
6*His
ERG (M1-Y486)
Accession # P11308-3
C-term
Synonyms
ERG3; included; ets related; ETS-related gene; KCNH2; Oncogene TMPRSS2/ERG fusion
AA Sequence

MIQTVPDPAAHIKEALSVVSEDQSLFECAYGTPHLAKTEMTASSSSDYGQTSKMSPRVPQQDWLSQPPARVTIKMECNPSQVNGSRNSPDECSVAKGGKMVGSPDTVGMNYGSYMEEKHMPPPNMTTNERRVIVPADPTLWSTDHVRQWLEWAVKEYGLPDVNILLFQNIDGKELCKMTKDDFQRLTPSYNADILLSHLHYLRETPLPHLTSDDVDKALQNSPRLMHARNTGGAAFIFPNTSVYPEATQRITTRPDLPYEPPRRSAWTGHGHPTPQSKAAQPSPSTVPKTEDQRPQLDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSSNSSCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPPESSLYKYPSDLPYMGSYHAHPQKMNFVAPHPPALPVTSSSFFAAPNPYWNSPTGGIYPNTRLPTSHMPSHLGTYY

Molecular Weight

Approximately 57 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ERG Protein, Human (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
ERG Protein, Human (P.pastoris, His)
Cat. No.:
HY-P71733
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