Erythropoietin/EPO Protein, Mouse (HEK293, solution)
Based on 1 Customer Validation
This gene encodes the erythropoietin (EPO) protein that is critical for regulating erythropoiesis. It is mainly expressed in the liver during fetal development and is transferred to the kidneys in adults. Erythropoietin/EPO Protein, Mouse (HEK293, solution) is the recombinant mouse-derived Erythropoietin/EPO protein, expressed by HEK293 , with tag free.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
This gene encodes the erythropoietin (EPO) protein that is critical for regulating erythropoiesis. It is mainly expressed in the liver during fetal development and is transferred to the kidneys in adults. Erythropoietin/EPO Protein, Mouse (HEK293, solution) is the recombinant mouse-derived Erythropoietin/EPO protein, expressed by HEK293 , with tag free.
Background
This gene encodes the glycoprotein hormone erythropoietin, which plays a crucial role in regulating red blood cell production and hemoglobin biosynthesis. While this gene is predominantly expressed in the liver during fetal development, it shifts to the kidneys in adults. Complete absence of the encoded protein leads to embryonic lethal anemia in mice, and conditional inactivation in adult mice results in chronic, normocytic, and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Various isoforms are generated through alternative splicing. Notably, the expression of this gene is low in the reference dataset.
Verified Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is typically 2-10 ng/mL.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag Tag Free
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Accession
NP_031968.1 (A27-R192)
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Molecular Construction
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N-term
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EPO (A27-R192)
Accession # NP_031968.1 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
EPO; ECYT5; Erythropoietin; MVCD2; EP; DBAL; Epoetin
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AA Sequence
APPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMEVEEQAIEVWQGLSLLSEAILQAQALLANSSQPPETLQLHIDKAISGLRSLTSLLRVLGAQKELMSPPDTTPPAPLRTLTVDTFCKLFRVYANFLRGKLKLYTGEVCRRGDR
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Predicted Molecular Mass
18.6 kDa
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Molecular Weight
Approximately 25-38 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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