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  3. Erythropoietin/EPO Protein, Mouse (HEK293, solution)

Erythropoietin/EPO Protein, Mouse (HEK293, solution)

Cat. No.: HY-P73038
COA Handling Instructions

This gene encodes the erythropoietin (EPO) protein that is critical for regulating erythropoiesis. It is mainly expressed in the liver during fetal development and is transferred to the kidneys in adults. Erythropoietin/EPO Protein, Mouse (HEK293, solution) is the recombinant mouse-derived Erythropoietin/EPO protein, expressed by HEK293 , with tag free. The total length of Erythropoietin/EPO Protein, Mouse (HEK293, solution) is 166 a.a., with molecular weight of ~18.6 kDa.

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Description

This gene encodes the erythropoietin (EPO) protein that is critical for regulating erythropoiesis. It is mainly expressed in the liver during fetal development and is transferred to the kidneys in adults. Erythropoietin/EPO Protein, Mouse (HEK293, solution) is the recombinant mouse-derived Erythropoietin/EPO protein, expressed by HEK293 , with tag free. The total length of Erythropoietin/EPO Protein, Mouse (HEK293, solution) is 166 a.a., with molecular weight of ~18.6 kDa.

Background

This gene encodes the glycoprotein hormone erythropoietin, which plays a crucial role in regulating red blood cell production and hemoglobin biosynthesis. While this gene is predominantly expressed in the liver during fetal development, it shifts to the kidneys in adults. Complete absence of the encoded protein leads to embryonic lethal anemia in mice, and conditional inactivation in adult mice results in chronic, normocytic, and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Various isoforms are generated through alternative splicing. Notably, the expression of this gene is low in the reference dataset.

Biological Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is typically 2-10 ng/mL.

Species

Mouse

Source

HEK293

Tag

Tag Free

Accession

NP_031968.1 (A27-R192)

Gene ID

13856  [NCBI]

Molecular Construction
N-term
EPO (A27-R192)
Accession # NP_031968.1
C-term
Synonyms
ECYT5; EP; EPO; epoetin; Erythropoietin; MVCD2
AA Sequence

MGVPERPTLLLLLSLLLIPLGLPVLCAPPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMEVEEQAIEVWQGLSLLSEAILQAQALLANSSQPPETLQLHIDKAISGLRSLTSLLRVLGAQKELMSPPDTTPPAPLRTLTVDTFCKLFRVYANFLRGKLKLYTGEVCRRGDR

Molecular Weight

Approximately 23-36 kDa due to glycosylation.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Erythropoietin/EPO Protein, Mouse (HEK293, solution) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Erythropoietin/EPO Protein, Mouse (HEK293, solution)
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HY-P73038
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