1. Recombinant Proteins
  2. Fc Receptors
  3. FcRn
  4. FCGRT-B2M Heterodimer Protein, Human (HEK293, His)

FCGRT-B2M Heterodimer Protein, Human (HEK293, His)

Cat. No.: HY-P73522
COA Handling Instructions

The FCGRT protein is a cell surface receptor that conveys passive humoral immunity through the selective uptake of monomeric IgG in milk. It binds IgG at the intestinal epithelium and forms an FcRn-IgG complex that is transcytosed across the epithelium, releasing IgG into the blood or tissue. FCGRT-B2M Heterodimer Protein, Human (HEK293, His) is a recombinant protein dimer complex containing human-derived FCGRT-B2M Heterodimer protein, expressed by HEK293 , with C-His labeled tag. FCGRT-B2M Heterodimer Protein, Human (HEK293, His), has molecular weight of ~35 & 12 kDa, respectively.

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  • Biological Activity

  • Technical Parameters

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Description

The FCGRT protein is a cell surface receptor that conveys passive humoral immunity through the selective uptake of monomeric IgG in milk. It binds IgG at the intestinal epithelium and forms an FcRn-IgG complex that is transcytosed across the epithelium, releasing IgG into the blood or tissue. FCGRT-B2M Heterodimer Protein, Human (HEK293, His) is a recombinant protein dimer complex containing human-derived FCGRT-B2M Heterodimer protein, expressed by HEK293 , with C-His labeled tag. FCGRT-B2M Heterodimer Protein, Human (HEK293, His), has molecular weight of ~35 & 12 kDa, respectively.

Background

FCGRT, a crucial cell surface receptor, facilitates the transfer of passive humoral immunity from the mother to the newborn by binding to the Fc region of monomeric immunoglobulin gamma and selectively mediating its uptake from milk. The process involves IgG binding at the apical surface of the intestinal epithelium, forming FcRn-IgG complexes that transcytose across the intestinal epithelium, releasing IgG into blood or tissue fluids. Beyond infancy, FCGRT plays a pivotal role in effective humoral immunity by recycling IgG and prolonging its half-life in the circulation. Mechanistically, the binding of monomeric IgG to FCGRT in acidic endosomes of endothelial and hematopoietic cells facilitates the recycling of IgG to the cell surface, subsequently releasing it into circulation. Additionally, FCGRT regulates the homeostasis of albumin/ALB, the other most abundant circulating protein, further underscoring its essential role in immune and protein homeostasis. In the context of microbial infection, FCGRT acts as an uncoating receptor for various echoviruses, including Echovirus 5, 6, 7, 9, 11, 13, 25, and 29.

Biological Activity

Measured by its ability to bind human IgG1 in a functional ELISA.

Species

Human

Source

HEK293

Tag

C-His

Accession

P55899 (A24-S297)&P61769 (I21-M119)

Gene ID

2217  [NCBI]&567

Synonyms
FCGRT-B2M Heterodimer Protein; IgG receptor FcRn large subunit p51; Beta-2-microglobulin
Molecular Weight

Approximately 35&12 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

FCGRT-B2M Heterodimer Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
FCGRT-B2M Heterodimer Protein, Human (HEK293, His)
Cat. No.:
HY-P73522
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