FGF basic Protein, Salmon
Based on 1 Customer Validation
FGF basic Protein, Salmon is the recombinant FGF-basic protein, expressed by E. coli, with tag free.
- Species: Others
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
FGF basic Protein, Salmon is the recombinant FGF-basic protein, expressed by E. coli, with tag free.
Measure by its ability by a dose-response proliferation assay using murine Balb/c 3T3 cells. The ED50 for this effect is <1 ng/mL. The specific activity of this protein is > 1.0 × 106 IU/mg. (It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.)
Technical Parameters
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Species Others
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Source E. coli
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Tag Tag Free
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Accession
XP_035600424.1 (M1-R155)
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Gene ID118363560
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Molecular Construction
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N-term
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FGF basic (M1-R155)
Accession # XP_035600424.1 -
C-term
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Protein Length
Full Length
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Synonyms
Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB
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AA Sequence
MATGEITTLPATPEDGGSGGFPPGNFKEPKRLYCKNGGYFLRINSNGSVDGIREKNDPHIKLQLQATSVGEVVIKGVSANRYLAMNGDGRLFGTRRTTDECYFMERLESNNYNTYRSRKYPDMYVALKRTGQHKSGSKTGPGQKAILFLPMSARR
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Molecular Weight
Approximately 20 kDa, based on SDS-PAGE under non reducing (N) conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.2 μm filtered solution of 7.8 mM Na2HPO4, 1.5 mM KH2PO4, 2.7 mM KCl, 500 mM NaCl.
< 0.2 EU/μg of protein by gel clotting method
It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (232 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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