FGFR-4 Protein, Mouse (HEK293, His)

FGFR-4 protein is a tyrosine protein kinase that serves as a cell surface receptor for fibroblast growth factor and controls proliferation, differentiation, migration, lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism, and phosphate and other cellular processes homeostasis. It significantly downregulates CYP7A1 in response to FGF19. FGFR-4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FGFR-4 protein, expressed by HEK293 , with C-His labeled tag.

FGFR-4 Protein is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors, regulating cell proliferation, differentiation, migration, lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism, and phosphate homeostasis. It is essential for the normal down-regulation of CYP7A1 expression, the enzyme involved in bile acid synthesis, in response to FGF19. Upon ligand binding, FGFR-4 phosphorylates PLCG1 and FRS2, leading to the activation of multiple signaling cascades. Activation of PLCG1 results in the production of diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 recruits GRB2, GAB1, PIK3R1, and SOS1, activating the RAS, MAPK1/ERK2, MAPK3/ERK1, and AKT1 signaling pathways. FGFR-4 also facilitates the SRC-dependent phosphorylation and lysosomal degradation of MMP14, a matrix protease, with MMP14 aiding in the internalization and degradation of FGFR-4. In addition, FGFR-4 plays a role in postnatal lung development and may be involved in skeletal muscle cell lineage development.

1.This product does not contain protein kinase domain.
2.Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ of this effect is 8.197 ng/ml in the presence of 2 ng/mL FGF-acidic, corresponding to a specific activity is 1.219×10⁵ units/mg.

• 
  Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ of this effect is 8.197 ng/mL in the presence of 2 ng/mL FGF-acidic, corresponding to a specific activity is 1.219×10⁵ units/mg.

Mouse

HEK293

C-6*His

Q03142/NP_032037.2 (L17-D366)

14186  [NCBI]

Extracellular Domain

LSLEASEEMEQEPCLAPILEQQEQVLTVALGQPVRLCCGRTERGRHWYKEGSRLASAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMTVVHNLTLLMDDSLTSISNDEDPKTLSSSSSGHVYPQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPMPTIHWLKDGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENSLGSIRYSYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCKVYSDAQPHIQWLKHVVINGSSFGADGFPYVQVLKTTDINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPEEDLTWTTATPEARYTD

Approximately 58-80 kDa due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.