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  3. FLT3LG Protein, Rat (sf9, His)

FLT3LG Protein, Rat (sf9, His)

Cat. No.: HY-P74150
Handling Instructions

The FLT3LG protein has the same protein-binding and receptor tyrosine kinase-binding activities predicted to positively regulate natural killer cell proliferation. Intended to be located on the cell surface and extracellular space, it serves as an intrinsic component on the outside of the plasma membrane. FLT3LG Protein, Rat (sf9, His) is the recombinant rat-derived FLT3LG protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of FLT3LG Protein, Rat (sf9, His) is 162 a.a., with molecular weight of ~19.7 kDa.

For research use only. We do not sell to patients.

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Description

The FLT3LG protein has the same protein-binding and receptor tyrosine kinase-binding activities predicted to positively regulate natural killer cell proliferation. Intended to be located on the cell surface and extracellular space, it serves as an intrinsic component on the outside of the plasma membrane. FLT3LG Protein, Rat (sf9, His) is the recombinant rat-derived FLT3LG protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of FLT3LG Protein, Rat (sf9, His) is 162 a.a., with molecular weight of ~19.7 kDa.

Background

The FLT3LG protein is predicted to possess identical protein binding activity and receptor tyrosine kinase binding activity. It plays a role in the positive regulation of natural killer cell proliferation and is anticipated to be located in the cell surface and extracellular space, serving as an intrinsic component of the external side of the plasma membrane. This protein has been utilized in the study of pancreatic cancer. In the context of human diseases, orthologs of this gene are implicated in colon carcinoma, glioblastoma, and high-grade glioma. The FLT3LG gene is orthologous to the human FLT3LG gene (fms related receptor tyrosine kinase 3 ligand). [provided by Alliance of Genome Resources, Apr 2022]

Species

Rat

Source

Sf9 insect cells

Tag

C-His

Accession

NP_001292868 (T28-Q189)

Gene ID

103691134  [NCBI]

Molecular Construction
N-term
FLT3LG (T28-Q189)
Accession # NP_001292868
His
C-term
Synonyms
Fms-related tyrosine kinase 3 ligand; Flt3 Ligand; Flt3L; SL Cytokine; FLT3LG
Molecular Weight

Approximately 19.7 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

FLT3LG Protein, Rat (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FLT3LG Protein, Rat (sf9, His)
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HY-P74150
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