G-CSFR/CD114 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
The G-CSFR/CD114 protein serves as a receptor for granulocyte colony-stimulating factor (CSF3) and plays a key role in granulocyte development and function. As a homodimer, it binds two CSF3 molecules and transduces signals. G-CSFR/CD114 Protein, Mouse (HEK293, His) is the recombinant mouse-derived G-CSFR/CD114 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The G-CSFR/CD114 protein serves as a receptor for granulocyte colony-stimulating factor (CSF3) and plays a key role in granulocyte development and function. As a homodimer, it binds two CSF3 molecules and transduces signals. G-CSFR/CD114 Protein, Mouse (HEK293, His) is the recombinant mouse-derived G-CSFR/CD114 protein, expressed by HEK293 , with C-His labeled tag.
Background
G-CSFR/CD114 Protein acts as the receptor for granulocyte colony-stimulating factor (CSF3) and may additionally play a role in certain adhesion or recognition events at the cell surface. Existing as a homodimer, the receptor binds two CSF3 molecules, facilitating the transduction of signals related to granulocyte development and function. Moreover, G-CSFR/CD114 interacts with CEACAM1, leading to the down-regulation of the CSF3R-STAT3 pathway. This process involves the recruitment of PTPN6, which subsequently dephosphorylates CSF3R, revealing a regulatory aspect of G-CSFR/CD114 in modulating signaling pathways associated with granulocyte colony-stimulating factor responses. These interactions highlight the intricate regulatory roles of G-CSFR/CD114 in cellular processes related to immune responses and hematopoiesis.
Verified Bioactivity
Immobilized Mouse G-CSF R, His Tag at 0.2 μg/mL (100 μl/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of ≤3 ng/mL determined by ELISA.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-8*His
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Accession
P40223 (C26-D626)
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Molecular Construction
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N-term
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CSF3R (C26-D626)
Accession # P40223 -
8*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
CSF3R; GCSFR; Prev. CD114; G-CSF Receptor; Granulocyte Colony-Stimulating Factor Receptor; CD114 Antigen; G-CSF-R; SCN7; Colony Stimulating Factor 3 Receptor; Colony Stimulating Factor 3 Receptor (Granulocyte)
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AA Sequence
CGHIEISPPVVRLGDPVLASCTISPNCSKLDQQAKILWRLQDEPIQPGDRQHHLPDGTQESLITLPHLNYTQAFLFCLVPWEDSVQLLDQAELHAGYPPASPSNLSCLMHLTTNSLVCQWEPGPETHLPTSFILKSFRSRADCQYQGDTIPDCVAKKRQNNCSIPRKNLLLYQYMAIWVQAENMLGSSESPKLCLDPMDVVKLEPPMLQALDIGPDVVSHQPGCLWLSWKPWKPSEYMEQECELRYQPQLKGANWTLVFHLPSSKDQFELCGLHQAPVYTLQMRCIRSSLPGFWSPWSPGLQLRPTMKAPTIRLDTWCQKKQLDPGTVSVQLFWKPTPLQEDSGQIQGYLLSWSSPDHQGQDIHLCNTTQLSCIFLLPSEAQNVTLVAYNKAGTSSPTTVVFLENEGPAVTGLHAMAQDLNTIWVDWEAPSLLPQGYLIEWEMSSPSYNNSYKSWMIEPNGNITGILLKDNINPFQLYRITVAPLYPGIVGPPVNVYTFAGERAPPHAPALHLKHVGTTWAQLEWVPEAPRLGMIPLTHYTIFWADAGDHSFSVTLNISLHDFVLKHLEPASLYHVYLMATSRAGSTNSTGLTLRTLDPSD
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Predicted Molecular Mass
68.5 kDa
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Molecular Weight
Approximately 90-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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