Galectin-1/LGALS1 Protein, Human (His)
Based on 1 Customer Validation
Galectin-1/LGALS1 is a lectin that binds β-galactoside and complex carbohydrates and regulates apoptosis, proliferation, and differentiation. It inhibits CD45 phosphatase activity, blocks Lyn kinase dephosphorylation, and induces T cell apoptosis. Galectin-1/LGALS1 Protein, Human (His) is the recombinant human-derived Galectin-1/LGALS1 protein, expressed by E. coli , with C-6*His labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Galectin-1/LGALS1 is a lectin that binds β-galactoside and complex carbohydrates and regulates apoptosis, proliferation, and differentiation. It inhibits CD45 phosphatase activity, blocks Lyn kinase dephosphorylation, and induces T cell apoptosis. Galectin-1/LGALS1 Protein, Human (His) is the recombinant human-derived Galectin-1/LGALS1 protein, expressed by E. coli , with C-6*His labeled tag.
Background
The Galectin-1/LGALS1 protein serves as a lectin with the ability to bind beta-galactoside and a diverse range of complex carbohydrates. It plays a pivotal role in the regulation of apoptosis, cell proliferation, and cell differentiation. Galectin-1/LGALS1 exerts its influence by inhibiting the protein phosphatase activity of CD45, consequently impeding the dephosphorylation of Lyn kinase. Additionally, it acts as a potent inducer of T-cell apoptosis. Existing as a homodimer, Galectin-1/LGALS1 forms interactions with a variety of cellular entities, including CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, and CD45. It also binds LGALS3BP, laminin (via poly-N-acetyllactosamine), and SUSD2. Notably, Galectin-1/LGALS1 engages in an interaction with the cargo receptor TMED10, facilitating translocation from the cytoplasm into the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and subsequent secretion.
Verified Bioactivity
CD45 immobilized on CM5 Chip can bind LGALS1 with an affinity constant of 4.859 μM as determined in SPR assay.
MCE Validation Data
-
Purity - SDS-PAGE
Purity - SDS-PAGE
-
Bioactivity - SPR
Bioactivity - SPR
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag C-6*His
-
Accession
P09382 (A2-D135)
-
Molecular Construction
-
N-term
-
LGALS1 (A2-D135)
Accession # P09382 -
6*His
-
C-term
-
-
Protein Length
Full Length
-
Synonyms
LGALS1; Galaptin; Galectin 1; HLBP14; Lectin, Galactoside-Binding, Soluble, 1; Gal-1; GBP; HBL; Beta-Galactoside-Binding Lectin L-14-I; HPL; Putative MAPK-Activating Protein PM12; Epididymis Secretory Sperm Binding Protein; 14 KDa Laminin-Binding Protein;
-
AA Sequence
ACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD
-
Molecular Weight
Approximately 15 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 20 mM Citrate, 8% trehalose, 4% mannitol, 50 mM NaCl, 1 mM TCEP, 0.05% Tween 80, pH 4.0.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM Citrate, 8% trehalose, 4% mannitol, 50 mM NaCl, 1mM TCEP, 0.05% Tween 80, pH 4.0.
3.Lyophilized from a 0.22 μm filtered solution of 20 mM Citrate, 8% trehalose, 4% mannitol, 50 mM NaCl, 0.05% Tween 80, 1 mM TCEP, pH 4.0.
4.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 1 mM TCEP, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (236 KB)
-
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
