Galectin-1/LGALS1 Protein, Rat

Galectin-1/LGALS1 protein is a β-galactoside-binding lectin that regulates apoptosis, cell proliferation and differentiation by inhibiting CD45 protein phosphatase activity and preventing Lyn kinase dephosphorylation. As a homodimer, it induces T cell apoptosis and interacts with CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, CD45, laminin, and SUSD2. Galectin-1/LGALS1 Protein, Rat is the recombinant rat-derived Galectin-1/LGALS1 protein, expressed by E. coli , with tag free.

Galectin-1/LGALS1 Protein is a lectin with a high affinity for binding beta-galactoside and a diverse range of complex carbohydrates. This protein serves a crucial role in the regulation of apoptosis, cell proliferation, and cell differentiation. Notably, it inhibits the activity of CD45 protein phosphatase, leading to the prevention of Lyn kinase dephosphorylation. Galectin-1/LGALS1 is a potent inducer of T-cell apoptosis. Existing as a homodimer, it forms interactions with various proteins, including CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, and CD45. Additionally, Galectin-1/LGALS1 interacts with laminin through poly-N-acetyllactosamine binding and associates with SUSD2. Furthermore, it engages in a notable interaction with the cargo receptor TMED10, facilitating translocation from the cytoplasm into the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and subsequent secretion.

Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ for this effect is 0.4036 μg/mL, corresponding to a specific activity is 2.477×10^3 units/mg.

• 
  Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ for this effect is 0.4036 μg/mL, corresponding to a specific activity is 2.477×10^3 units/mg.

Rat

E. coli

Tag Free

P11762 (A2-E135)

56646  [NCBI]

Full Length of Mature Protein

ACGLVASNLNLKPGECLKVRGELAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDDGTWGTEQRETAFPFQPGSITEVCITFDQADLTIKLPDGHEFKFPNRLNMEAINYMAADGDFKIKCVAFE

Approximately 13 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.