Galectin-1/LGALS1 Protein, Rat
Based on 1 Customer Validation
Galectin-1/LGALS1 protein is a β-galactoside-binding lectin that regulates apoptosis, cell proliferation and differentiation by inhibiting CD45 protein phosphatase activity and preventing Lyn kinase dephosphorylation. As a homodimer, it induces T cell apoptosis and interacts with CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, CD45, laminin, and SUSD2. Galectin-1/LGALS1 Protein, Rat is the recombinant rat-derived Galectin-1/LGALS1 protein, expressed by E. coli , with tag free.
- Species: Rat
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Galectin-1/LGALS1 protein is a β-galactoside-binding lectin that regulates apoptosis, cell proliferation and differentiation by inhibiting CD45 protein phosphatase activity and preventing Lyn kinase dephosphorylation. As a homodimer, it induces T cell apoptosis and interacts with CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, CD45, laminin, and SUSD2. Galectin-1/LGALS1 Protein, Rat is the recombinant rat-derived Galectin-1/LGALS1 protein, expressed by E. coli , with tag free.
Galectin-1/LGALS1 Protein is a lectin with a high affinity for binding beta-galactoside and a diverse range of complex carbohydrates. This protein serves a crucial role in the regulation of apoptosis, cell proliferation, and cell differentiation. Notably, it inhibits the activity of CD45 protein phosphatase, leading to the prevention of Lyn kinase dephosphorylation. Galectin-1/LGALS1 is a potent inducer of T-cell apoptosis. Existing as a homodimer, it forms interactions with various proteins, including CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, and CD45. Additionally, Galectin-1/LGALS1 interacts with laminin through poly-N-acetyllactosamine binding and associates with SUSD2. Furthermore, it engages in a notable interaction with the cargo receptor TMED10, facilitating translocation from the cytoplasm into the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and subsequent secretion.
Measured in a cell proliferation assay using HUVEC cells. The ED50 for this effect is 0.4036 μg/mL, corresponding to a specific activity is 2.477×10^3 units/mg.
Technical Parameters
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Species Rat
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Source E. coli
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Tag Tag Free
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Accession
P11762 (A2-E135)
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Molecular Construction
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N-term
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LGALS1 (A2-E135)
Accession # P11762 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
LGALS1; Galaptin; Galectin 1; HLBP14; Lectin, Galactoside-Binding, Soluble, 1; Gal-1; GBP; HBL; Beta-Galactoside-Binding Lectin L-14-I; HPL; Putative MAPK-Activating Protein PM12; Epididymis Secretory Sperm Binding Protein; 14 KDa Laminin-Binding Protein;
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AA Sequence
ACGLVASNLNLKPGECLKVRGELAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDDGTWGTEQRETAFPFQPGSITEVCITFDQADLTIKLPDGHEFKFPNRLNMEAINYMAADGDFKIKCVAFE
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Molecular Weight
Approximately 16 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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