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Gamma-enolase/Enolase 2 Protein, Human (His)

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Gamma-enolase/Enolase 2 protein exhibits neurotrophic and neuroprotective properties, binding to neocortical neurons and promoting cell survival through a calcium-dependent mechanism. This suggests its potential role in supporting neuron viability within the central nervous system. Gamma-enolase/Enolase 2 Protein, Human (His) is the recombinant human-derived Gamma-enolase/Enolase 2 protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

Gamma-enolase/Enolase 2 protein exhibits neurotrophic and neuroprotective properties, binding to neocortical neurons and promoting cell survival through a calcium-dependent mechanism. This suggests its potential role in supporting neuron viability within the central nervous system. Gamma-enolase/Enolase 2 Protein, Human (His) is the recombinant human-derived Gamma-enolase/Enolase 2 protein, expressed by E. coli , with N-6*His labeled tag.

Background

The Gamma-enolase/Enolase 2 protein manifests neurotrophic and neuroprotective properties across a diverse range of central nervous system (CNS) neurons. Through a calcium-dependent mechanism, it binds to cultured neocortical neurons, effectively promoting cell survival. These findings suggest a potential role for Gamma-enolase/Enolase 2 in supporting the viability of neurons within the central nervous system.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    P09104-1 (M1-L434)

  • Gene ID
    2026  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • ENO2 (M1-L434)
      Accession # P09104-1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    ENO2; Neural Enolase; Enolase 2; HEL-S-279; NSE; Epididymis Secretory Protein Li 279; 2-Phospho-D-Glycerate Hydro-Lyase; 2-Phospho-D-Glycerate Hydrolyase; Gamma-Enolase; Neuron Specific Gamma Enolase; Enolase 2 (Gamma, Neuronal); Phosphopyruvate Hydratase

  • AA Sequence

    MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEARFAGHNFRNPSVL

  • Predicted Molecular Mass

    49.4 kDa

  • Molecular Weight

    Approximately 46-55 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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