GARP&Latent TGF beta Complex Protein, Mouse (HEK293, His)

GARP/latent TGF-b1 complexes are potent inducers of Th17 differentiation in the presence of exogenous IL-6 and inducers of Treg in the presence of IL-2. GARP&Latent TGF beta Complex Protein, Mouse (HEK293, His) is a biotinylated recombinant protein (GARP (I18-N628)&TGF-b1 (L30-S390)) produced by HEK293 cells with His tag^[1][2].

GARP/latent TGF-b1 complexes are potent inducers of Th17 differentiation in the presence of exogenous IL-6 and inducers of Treg in the presence of IL-2^[1][2].
GARP/LTGFβ1 complex is expressed at very low levels on freshly explanted Treg, but markedly upregulated following Treg activation in vitro. GARP/Latent TGF-β1 complex on Treg cells modulates the induction of peripherally derived Treg cells during oral tolerance^[3]

Mouse

HEK293

N-8*His

G3XA59 (A18-L628)&P04202 (L30-S390)

434215  [NCBI]&21803  [NCBI]

70-80 kDa

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Soyoung A Oh,TGF-β: guardian of T cell function. J Immunol. 2013 Oct 15;191(8):3973-9.  [Content Brief]

  [2]. Angela X Zhou, et al. GARP-TGF-β complexes negatively regulate regulatory T cell development and maintenance of peripheral CD4+ T cells in vivo. J Immunol. 2013 May 15;190(10):5057-64.  [Content Brief]

  [3]. Justin P Edwards, et al. The GARP/Latent TGF-β1 complex on Treg cells modulates the induction of peripherally derived Treg cells during oral tolerance. Eur J Immunol. 2016 Jun;46(6):1480-9.  [Content Brief]

  [4]. Dulce Maroni, et al. TGFB1 disrupts the angiogenic potential of microvascular endothelial cells of the corpus luteum. Cell Sci. 2011 Jul 15;124(Pt 14):2501-10.  [Content Brief]

  [5]. Justin Rustenhoven, et al. TGF-beta1 regulates human brain pericyte inflammatory processes involved in neurovasculature function. Justin Rustenhoven. 2016 Feb 11;13:37.  [Content Brief]

  [6]. Kai Zhang, et al. Essential role of microglial transforming growth factor-β1 in antidepressant actions of (R)-ketamine and the novel antidepressant TGF-β1. Transl Psychiatry. 2020 Jan 27;10(1):32.  [Content Brief]