GARP&Latent TGF beta Complex Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
GARP/latent TGF-b1 complexes are potent inducers of Th17 differentiation in the presence of exogenous IL-6 and inducers of Treg in the presence of IL-2. GARP&Latent TGF beta Complex Protein, Mouse (HEK293, His) is a biotinylated recombinant protein (GARP (I18-N628)&TGF-b1 (L30-S390)) produced by HEK293 cells with His tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
GARP/latent TGF-b1 complexes are potent inducers of Th17 differentiation in the presence of exogenous IL-6 and inducers of Treg in the presence of IL-2. GARP&Latent TGF beta Complex Protein, Mouse (HEK293, His) is a biotinylated recombinant protein (GARP (I18-N628)&TGF-b1 (L30-S390)) produced by HEK293 cells with His tag[1][2].
Background
GARP/latent TGF-b1 complexes are potent inducers of Th17 differentiation in the presence of exogenous IL-6 and inducers of Treg in the presence of IL-2[1][2].
GARP/LTGFβ1 complex is expressed at very low levels on freshly explanted Treg, but markedly upregulated following Treg activation in vitro. GARP/Latent TGF-β1 complex on Treg cells modulates the induction of peripherally derived Treg cells during oral tolerance[3]
Technical Parameters
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Species Mouse
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Source HEK293
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Tag N-8*His
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Synonyms
LRRC32; Transforming Growth Factor Beta Activator LRRC32; Prev. D11S833E; Garpin; Prev. GARP; CPPRDD; Glycoprotein A Repetitions Predominant; Leucine Rich Repeat Containing 32; Leucine-Rich Repeat-Containing Protein 32; TGFB1; Transforming Growth Factor B
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Predicted Molecular Mass
66.6 kDa (GARP) & 42.4 kDa (Latent TGF beta 1)
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Molecular Weight
Approximately 70-80 kDa (GARP) & 13 kDa & 42-50 kDa (Latent TGF beta 1), based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Soyoung A Oh,TGF-β: guardian of T cell function. J Immunol. 2013 Oct 15;191(8):3973-9. [Content Brief]
[2]. Angela X Zhou, et al. GARP-TGF-β complexes negatively regulate regulatory T cell development and maintenance of peripheral CD4+ T cells in vivo. J Immunol. 2013 May 15;190(10):5057-64. [Content Brief]
[3]. Justin P Edwards, et al. The GARP/Latent TGF-β1 complex on Treg cells modulates the induction of peripherally derived Treg cells during oral tolerance. Eur J Immunol. 2016 Jun;46(6):1480-9. [Content Brief]
[4]. Dulce Maroni, et al. TGFB1 disrupts the angiogenic potential of microvascular endothelial cells of the corpus luteum. Cell Sci. 2011 Jul 15;124(Pt 14):2501-10. [Content Brief]
[5]. Justin Rustenhoven, et al. TGF-beta1 regulates human brain pericyte inflammatory processes involved in neurovasculature function. Justin Rustenhoven. 2016 Feb 11;13:37. [Content Brief]
[6]. Kai Zhang, et al. Essential role of microglial transforming growth factor-β1 in antidepressant actions of (R)-ketamine and the novel antidepressant TGF-β1. Transl Psychiatry. 2020 Jan 27;10(1):32. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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