GARP (Mutated)&Latent TGF Beta Complex Protein, Human (HEK293, His-Avi)
LRRC32 is a key regulator of TGF-β activation and maintains TGFB1, TGFB2, and TGFB3 in the latent state during extracellular storage by binding to latency-associated peptide (LAP). LRRC32 competes with LTBP1 for LAP binding and effectively regulates integrin-dependent TGF-β activation. GARP (Mutated) &Latent TGF Beta Complex Protein, Human (HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived GARP, expressed by HEK293 , with C-Avi, C-His labeled tag. GARP (Mutated) &Latent TGF Beta Complex Protein, Human (HEK293, His-Avi), has molecular weight of 75-80 kDa.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
LRRC32 is a key regulator of TGF-β activation and maintains TGFB1, TGFB2, and TGFB3 in the latent state during extracellular storage by binding to latency-associated peptide (LAP). LRRC32 competes with LTBP1 for LAP binding and effectively regulates integrin-dependent TGF-β activation. GARP (Mutated) &Latent TGF Beta Complex Protein, Human (HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived GARP, expressed by HEK293 , with C-Avi, C-His labeled tag. GARP (Mutated) &Latent TGF Beta Complex Protein, Human (HEK293, His-Avi), has molecular weight of 75-80 kDa.
Background
LRRC32, a crucial regulator of transforming growth factor beta (TGFB1, TGFB2, and TGFB3), plays a pivotal role in controlling TGF-beta activation by maintaining it in a latent state during extracellular storage. Specifically associating with the Latency-associated peptide (LAP), the regulatory chain of TGF-beta, LRRC32 exerts its regulatory influence on integrin-dependent TGF-beta activation. Notably, LRRC32 competes effectively with LTBP1 for LAP binding, further modulating TGF-beta activation. Its significance extends to the regulation of TGF-beta-1 (TGFB1) activation on the surface of activated regulatory T-cells (Tregs). Moreover, LRRC32's involvement is essential for epithelial fusion during palate development, where it regulates the activation of TGF-beta-3 (TGFB3). Interacting directly with TGFB1, TGFB2, and TGFB3, LRRC32's association with LAP regulates the activation of TGF-beta-1 and TGF-beta-3, highlighting its intricate role in fine-tuning TGF-beta signaling. Additionally, LRRC32 interacts with LAPTM4B, contributing to the reduction of TGFB1 production in regulatory T-cells.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-8*His
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Synonyms
LRRC32; Transforming Growth Factor Beta Activator LRRC32; Prev. D11S833E; Garpin; Prev. GARP; CPPRDD; Glycoprotein A Repetitions Predominant; Leucine Rich Repeat Containing 32; Leucine-Rich Repeat-Containing Protein 32; TGFB1; Transforming Growth Factor B
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Predicted Molecular Mass
70.3 kDa (GARP(Y137H & S138G & G139N)) & 41.4 kDa (Latent TGF Beta 1)
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Molecular Weight
Approximately 75-80 kDa (GARP(Y137H & S138G & G139N)) & 15 kDa & 43-47 kDa (Latent TGF Beta 1), based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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