GH/Somatotropin Protein, Human
Based on 1 Customer Validation
GH Protein, Human is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration, which can be used for the study of growth disorders.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
GH Protein, Human is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration, which can be used for the study of growth disorders.
Recombinant Human Growth Hormone is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration, which can be used for the treatment of growth disorders in children[1]. Growth Hormone is a stress hormone that stimulates production of IGF-1 and raises the concentration of glucose and free fatty acids[2]. Growth Hormone exerts some of its effects by binding to receptors on target cells, where it activates the MAPK/ERK pathway[3].
ED50 < 0.5 ng/mL, measured by a cell proliferation assay using Nb2-11 Cells, corresponding to a specific activity of > 2.0 × 106 units/mg.
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Measured in a cell proliferation assay using Nb2-11 rat lymphoma cells.The ED50 for this effect is 0.0918 ng/mL, corresponding to a specific activity is 1.089×107 U/mg.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
P01241-1 (F27-F217)
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Molecular Construction
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N-term
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GH (F27-F217)
Accession # P01241-1 -
C-term
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
rHuGH; Somatotropin; GH-N; GH1; Growth hormone; Growth hormone 1; Pituitary growth hormone
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AA Sequence
FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF
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Molecular Weight
Approximately 20-22 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
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≥ 95%, as determined by reducing SDS-PAGE.
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Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of PBS.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
<1.0 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Takeda A, et al. Recombinant human growth hormone for the treatment of growth disorders in children: a systematic review and economic evaluation. Health Technol Assess. 2010 Sep;14(42):1-209, iii-iv. [Content Brief]
[2]. Ranabir S, et al. Stress and hormones. Indian J Endocrinol Metab. 2011 Jan;15(1):18-22. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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