IFN-alpha 2b/IFNA2 Protein, Human

Cat. No.: HY-P7023: Data Sheet
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IFN-alpha 2 (IFNA2), belongs to type I interferon family, is a protein secreted by cells infected by a virus and acting on other cells to inhibit viral infection. IFN-alpha 2 increases the frequencies of cytotoxic CD8⁺ T cells and induces CD4⁺ T cell depletion. IFN-alpha 2b/IFNA2 Protein, Human contains 165 a.a. (C24-E188), is a protein produced in E. coli with tag free.

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8 Publications Citing Use of MCE IFN-alpha 2b/IFNA2 Protein, Human

In Vivo Efficacy Study

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: IFN-alpha 2b/IFNA2 Protein, Human purchased from MCE. Usage Cited in: iScience. 2025 Jul 3;28(8):113053. [Abstract]; IFN-α (1000 U/mL; 7 days). Statistical analysis of the growth curve of tumors formed by BxPC-3 cells within 31 days was performed. After BxPC-3 cells formed tumors, CD8 tiselizumab with or without IFN-α and Tα1 for 7 days was administered to the mice via tail vein injection every 4 days.

: IFN-alpha 2b/IFNA2 Protein, Human purchased from MCE. Usage Cited in: iScience. 2025 Jul 3;28(8):113053. [Abstract]; IFN-α (1000 U/mL; 7 days). After BxPC3 cells formed tumors, CD8 tiselizumab with or without IFN-α and Tα1 was administered to the mice via tail vein injection every 4 days for 7 days. The protein level of CD8 in tumor specimens derived from 3 representative mice was examined by Western blotting.

: IFN-alpha 2b/IFNA2 Protein, Human purchased from MCE. Usage Cited in: iScience. 2025 Jul 3;28(8):113053. [Abstract]; IFN-α (1000 U/mL; 7 days). Statistical analysis of lysed PANC-1, BXPC-3, and MIAPaCa-2 cells after coculture with CD8+ T cells was determined by a CCK8 assay. The T cells were treated with 1 μg/mL of tislelizumab in the presence or absence of IFN-α and Tα1 for 7 days and then separated and cocultured with PDAC cells for 48 h.

: IFN-alpha 2b/IFNA2 Protein, Human purchased from MCE. Usage Cited in: iScience. 2025 Jul 3;28(8):113053. [Abstract]; IFN-α (1000 U/mL; 7 days). Statistical analysis of the levels of the secreted cytokines IL-2, TNF-α, and IFN-γ in suspensions of cultured CD8+ cells treated with 1 μg/mL of tislelizumab with or without the addition of IFN-α and Tα1 for 14 days was performed by ELISA.

: IFN-alpha 2b/IFNA2 Protein, Human purchased from MCE. Usage Cited in: iScience. 2025 Jul 3;28(8):113053. [Abstract]; IFN-α (1000 U/mL; 7 days). Flow cytometry analysis showed PD-1 levels on CD8 Tα1.

Biological Activity
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Description	IFN-alpha 2 (IFNA2), belongs to type I interferon family, is a protein secreted by cells infected by a virus and acting on other cells to inhibit viral infection^[1]. IFN-alpha 2 increases the frequencies of cytotoxic CD8⁺ T cells and induces CD4⁺ T cell depletion^[3]. IFN-alpha 2b/IFNA2 Protein, Human contains 165 a.a. (C24-E188), is a protein produced in E. coli with tag free.
Background	IFN-alpha 2 (IFNA2; IFN-α2), belongs to the type I interferon family, produced by the plasmacytoid dendritic cells (pDCs) exposure to HIV-1BaL in order to inhibit viral infection^[1]. Interferon (IFN) is originally identified as a substance ‘interfering’ with viral replication in vitro. IFN-α/β and related molecules are classified as type I IFNs, as for the other two types of type II IFN (IFN-γ) and type III IFNs (IFN-λ), respectively^[2]. IFN-alpha 2 subtype is the only one that is currently licensed to treat infections caused by hepatitis B virus (HBV) and HCV^[3]. IFN-alpha 2 shows a Sortilin-dependent trafficking in cells and increases the expression level of interferon-stimulated genes (ISGs) in HIV-infected cells^[1]^[4]. It also exhibits cytotoxic activity against CD8⁺ T cells and enhances CD4⁺ T cell depletion^[3]. Among the IFN-alpha 2 alleles, IFN-alpha 2b is being the predominant allele while IFNα-2a is less predominant and IFNα-2c only a minor allelic variant^[5]. IFN-alpha 2 has a bored application in research of cancer, including some hematological malignancies and solid tumors^[6].
In Vivo	IFNA2a (hydrodynamic injection with plasmids) leads to upregulation of interferon-stimulated genes (ISGs) and results HIV-1 induced CD4+ T cell depletion in the hu-PBL humanized mouse model^[3].
Biological Activity	1.The ED₅₀ is <0.4 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >2.5 × 10⁶ units/mg. 2.Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV) The ED₅₀ for this effect is <5 ng/mL. Measured by its ability to inhibit proliferation of TF-1 cells. The ED₅₀ for this effect is 0.06968 ng/mL, corresponding to a specific activity is 1.435×10⁷ units/mg.
Species	Human
Source	E. coli
Tag	Tag Free
Accession	P01563 (C24-E188)
Gene ID	3440 [NCBI]
Molecular Construction	N-term IFNA2 (C24-E188) Accession # P01563 C-term
Protein Length	Full Length of Mature Protein
Synonyms	rHuIFN-α2b; IFNA; INFA2; IFN-a 2b
AA Sequence	CDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE
Predicted Molecular Mass	19.4 kDa
Molecular Weight	Approximately 17-20 kDa, based on SDS-PAGE under reducing conditions.
Purity	≥ 95%, as determined by reducing SDS-PAGE.
Appearance	Lyophilized powder
Formulation	1.Lyophilized from a 0.22 μm filtered solution of PBS pH 7.4. 2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2. 3.Lyophilized from a 0.22 μm filtered solution of PBS, 8% trehalose, pH 7.4.
Endotoxin Level	<1 EU/μg, determined by LAL method.
Reconstitution	It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Storage & Stability	Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping	Room temperature in continental US; may vary elsewhere.
Documentation	Select Batch: Data Sheet (265 KB) SDS (252 KB) COA (220 KB) Handling Instructions (2659 KB)
References	[1]. Abraham S, et al. Gene therapy with plasmids encoding IFN-β or IFN-α14 confers long-term resistance to HIV-1 in humanized mice. Oncotarget. 2016 Nov 29;7(48):78412-78420. [Content Brief] [2]. Gerasymenko IM, et al. MULTIPLEX PCR ASSAY FOR DETECTION OF HUMAN SOMATOTROPIN AND INTERFERON ALPHA2b GENES IN PLANT MATERIAL. Tsitol Genet. 2015 May-Jun;49(3):3-8. [Content Brief] [3]. Zhang SY, et al. Inborn errors of interferon (IFN)-mediated immunity in humans: insights into the respective roles of IFN-alpha/beta, IFN-gamma, and IFN-lambda in host defense. Immunol Rev. 2008 Dec;226:29-40. [Content Brief] [4]. Chang CH, et al. A new method to produce monoPEGylated dimeric cytokines shown with human interferon-α2b. Bioconjug Chem. 2009 Oct 21;20(10):1899-907. [Content Brief] [5]. Sutter K, et al. Interferon α subtypes in HIV infection. Cytokine Growth Factor Rev. 2018 Apr;40:13-18. [Content Brief] [6]. Watanabe H, et al. Detailed structure of mouse interferon α2 and its interaction with Sortilin. J Biochem. 2021 Oct 11;170(2):265-273. [Content Brief] [7]. Gull I, et al. Heterologous expression, immunochemical and computational analysis of recombinant human interferon alpha 2b. Springerplus. 2013 Jun 15;2(1):264. [Content Brief] [8]. Paul F, et al. IFNA2: The prototypic human alpha interferon. Gene. 2015 Aug 10;567(2):132-7. [Content Brief]