1. Recombinant Proteins
  2. Receptor Proteins
  3. G-Protein-Coupled Receptors (GPCRs)
  4. Adhesion GPCRs
  5. Adhesion G Protein-Coupled Receptor D1 (GPR133)
  6. GPR133 Protein, Human (HEK293, His)

GPR133 Protein, Human (HEK293, His)

Cat. No.: HY-P76961
COA Handling Instructions

GPR133 Protein, an orphan receptor, signals through the G(s)-alpha family of G-proteins, as indicated by studies. Its protumorigenic function, particularly in glioblastoma, suggests a potential role in tumorigenesis-associated pathological processes. GPR133 Protein, Human (HEK293, His) is the recombinant human-derived GPR133 protein, expressed by HEK293 , with C-His labeled tag. The total length of GPR133 Protein, Human (HEK293, His) is 545 a.a., with molecular weight of 90-110 KDa.

For research use only. We do not sell to patients.

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Description

GPR133 Protein, an orphan receptor, signals through the G(s)-alpha family of G-proteins, as indicated by studies. Its protumorigenic function, particularly in glioblastoma, suggests a potential role in tumorigenesis-associated pathological processes. GPR133 Protein, Human (HEK293, His) is the recombinant human-derived GPR133 protein, expressed by HEK293 , with C-His labeled tag. The total length of GPR133 Protein, Human (HEK293, His) is 545 a.a., with molecular weight of 90-110 KDa.

Background

GPR133 Protein, an orphan receptor, operates as a signaling entity through the G(s)-alpha family of G-proteins, as evidenced by studies. Notably, it exhibits a protumorigenic function, particularly underscored in glioblastoma, signifying its potential role in pathological processes associated with tumorigenesis.

Species

Human

Source

HEK293

Tag

C-10*His

Accession

Q6QNK2-1/NP_942122.2 (V26-S570)

Gene ID
Molecular Construction
N-term
GPR133 (V26-S570)
Accession # Q6QNK2/NP_942122.2
His
C-term
Synonyms
Adhesion G-protein coupled receptor D1; G-protein coupled receptor 133; ADGRD1; PGR25
AA Sequence

VYSRSQDHPGFQVLASASHYWPLENVDGIHELQDTTGDIVEGKVNKGIYLKEEKGVTLLYYGRYNSSCISKPEQCGPEGVTFSFFWKTQGEQSRPIPSAYGGQVISNGFKVCSSGGRGSVELYTRDNSMTWEASFSPPGPYWTHVLFTWKSKEGLKVYVNGTLSTSDPSGKVSRDYGESNVNLVIGSEQDQAKCYENGAFDEFIIWERALTPDEIAMYFTAAIGKHALLSSTLPSLFMTSTASPVMPTDAYHPIITNLTEERKTFQSPGVILSYLQNVSLSLPSKSLSEQTALNLTKTFLKAVGEILLLPGWIALSEDSAVVLSLIDTIDTVMGHVSSNLHGSTPQVTVEGSSAMAEFSVAKILPKTVNSSHYRFPAHGQSFIQIPHEAFHRHAWSTVVGLLYHSMHYYLNNIWPAHTKIAEAMHHQDCLLFATSHLISLEVSPPPTLSQNLSGSPLITVHLKHRLTRKQHSEATNSSNRVFVYCAFLDFSSGEGVWSNHGCALTRGNLTYSVCRCTHLTNFAILMQVVPLELARGHQVALSSIS

Molecular Weight

Approximately 90-110 kDa due to the glycosylation.

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
GPR133 Protein, Human (HEK293, His)
Cat. No.:
HY-P76961
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