GRK2 Protein, Human (sf9, His-GST)
The GRK2 protein is a highly specific, selective phosphorylation agonist that occupies beta-adrenergic receptors and related receptors, inducing desensitization and serving as an important regulatory mechanism. In addition to G protein-coupled receptor desensitization, GRK2 actively regulates the ciliary smoothing (SMO)-dependent Hedgehog (Hh) pathway by promoting SMO transport and enhancing SMO activity. GRK2, Human (sf9, His-GST) is the recombinant human-derived GRK2 protein, expressed by sf9 insect cells, with N-Avi labeled tag.
- Species: Human
- Source: sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
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Biological Activity
Description
The GRK2 protein is a highly specific, selective phosphorylation agonist that occupies beta-adrenergic receptors and related receptors, inducing desensitization and serving as an important regulatory mechanism. In addition to G protein-coupled receptor desensitization, GRK2 actively regulates the ciliary smoothing (SMO)-dependent Hedgehog (Hh) pathway by promoting SMO transport and enhancing SMO activity. GRK2, Human (sf9, His-GST) is the recombinant human-derived GRK2 protein, expressed by sf9 insect cells, with N-Avi labeled tag.
Background
GRK2 protein demonstrates a high degree of specificity as it selectively phosphorylates the agonist-occupied form of beta-adrenergic and closely related receptors, provoking their desensitization. This phosphorylation activity serves as a pivotal regulatory mechanism in receptor signaling. Additionally, GRK2 acts as a key modulator in LPAR1 signaling, engaging in competition with RALA for LPAR1 binding and influencing the receptor's signaling properties. Notably, GRK2 exhibits a phosphorylation-independent desensitization of LPAR1 and LPAR2. Beyond its role in G protein-coupled receptor desensitization, GRK2 plays a significant positive regulatory role in the ciliary Smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating SMO trafficking into the cilium and enhancing SMO activity. Furthermore, GRK2's involvement in the regulation of airway smooth muscle relaxation in response to blue light adds another layer to its diverse functional repertoire.
Technical Parameters
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Species Human
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Source sf9 insect cells
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Tag N-His;N-GST
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Accession
P25098 (A2-L689)
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Gene ID156
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Molecular Construction
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N-term
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His-GST
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(A2-L689)
Accession # P25098 -
C-term
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Protein Length
Full Length
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Synonyms
GRK2; Adrenergic, Beta, Receptor Kinase 1; Prev. ADRBK1; Adrenergic Beta Receptor Kinase 1; BARK1; G Protein-Coupled Receptor Kinase; Beta-Adrenergic Receptor Kinase 1; BETA-ARK1; Beta-ARK-1; BARK; G Protein-Coupled Receptor Kinase 2; G-Protein Coupled Re
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AA Sequence
ADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipped with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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