HABP2 Protein, Human (HEK293, His)

HABP2 is a protein that cleaves the alpha chain of fibrinogen as well as the beta chain between "Lys-53" and "Lys-54" at multiple sites, preventing direct formation of a fibrin clot. It activates coagulation factor VII and converts prourokinase into its active form. HABP2 Protein, Human (HEK293, His) is the recombinant human-derived HABP2 protein, expressed by HEK293 , with C-6*His labeled tag.

HABP2, a multifunctional protein, exhibits proteolytic activity by cleaving the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' of fibrinogen, while sparing the gamma-chain, thereby preventing direct initiation of fibrin clot formation and fibrinolysis. Notably, HABP2 does not activate prothrombin and plasminogen but facilitates the conversion of pro-urokinase to its active two-chain form. Additionally, it serves as an activator of coagulation factor VII. Beyond its role in hemostasis, HABP2 may act as a tumor suppressor, exerting negative regulation on cell proliferation and migration. Structurally, HABP2 exists as a heterodimer, with a 50 kDa heavy chain and a 27 kDa light chain linked by a disulfide bond.

Human

HEK293

C-6*His

Q14520-1 (F24-Q279)

3026  [NCBI]

Partial

FSLMSLLESLDPDWTPDQYDYSYEDYNQEENTSSTLTHAENPDWYYTEDQADPCQPNPCEHGGDCLVHGSTFTCSCLAPFSGNKCQKVQNTCKDNPCGRGQCLITQSPPYYRCVCKHPYTGPSCSQVVPVCRPNPCQNGATCSRHKRRSKFTCACPDQFKGKFCEIGSDDCYVGDGYSYRGKMNRTVNQHACLYWNSHLLLQENYNMFMEDAETHGIGEHNFCRNPDADEKPWCFIKVTNDKVKWEYCDVSACSAQ

35-45 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/sf9-cell.html  [Content Brief]