HABP2 Protein, Human (HEK293, His)
Based on 2 publication(s) in Google Scholar
HABP2 is a protein that cleaves the alpha chain of fibrinogen as well as the beta chain between "Lys-53" and "Lys-54" at multiple sites, preventing direct formation of a fibrin clot. It activates coagulation factor VII and converts prourokinase into its active form. HABP2 Protein, Human (HEK293, His) is the recombinant human-derived HABP2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
HABP2 is a protein that cleaves the alpha chain of fibrinogen as well as the beta chain between "Lys-53" and "Lys-54" at multiple sites, preventing direct formation of a fibrin clot. It activates coagulation factor VII and converts prourokinase into its active form. HABP2 Protein, Human (HEK293, His) is the recombinant human-derived HABP2 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
HABP2, a multifunctional protein, exhibits proteolytic activity by cleaving the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' of fibrinogen, while sparing the gamma-chain, thereby preventing direct initiation of fibrin clot formation and fibrinolysis. Notably, HABP2 does not activate prothrombin and plasminogen but facilitates the conversion of pro-urokinase to its active two-chain form. Additionally, it serves as an activator of coagulation factor VII. Beyond its role in hemostasis, HABP2 may act as a tumor suppressor, exerting negative regulation on cell proliferation and migration. Structurally, HABP2 exists as a heterodimer, with a 50 kDa heavy chain and a 27 kDa light chain linked by a disulfide bond.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Publications (2)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
FSAP aggravated endothelial dysfunction and neurological deficits in acute ischemic stroke due to large vessel occlusion. [Abstract]2022 Jan 7;7(1):6. PMID: 34992208 -
MedComm
Low-dose exercise protects the heart against established myocardial infarction via IGF-1-upregulated CTRP9 in male mice. [Abstract]2023 Nov 24;4(6):e411. PMID: 38020715
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q14520-1 (F24-Q279)
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Molecular Construction
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N-term
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HABP2 (F24-Q279)
Accession # Q14520-1 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
HABP2; Factor VII Activating Protein; Hyaluronan Binding Protein 2; Hyaluronan-Binding Protein 2; HGFAL; FVII Activator; PHBP; PHBSP; FSAP; Hyaluronic Acid Binding Protein 2; HABP; Plasma Hyaluronan-Binding Protein; Hepatocyte Growth Factor Activator-Like
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AA Sequence
FSLMSLLESLDPDWTPDQYDYSYEDYNQEENTSSTLTHAENPDWYYTEDQADPCQPNPCEHGGDCLVHGSTFTCSCLAPFSGNKCQKVQNTCKDNPCGRGQCLITQSPPYYRCVCKHPYTGPSCSQVVPVCRPNPCQNGATCSRHKRRSKFTCACPDQFKGKFCEIGSDDCYVGDGYSYRGKMNRTVNQHACLYWNSHLLLQENYNMFMEDAETHGIGEHNFCRNPDADEKPWCFIKVTNDKVKWEYCDVSACSAQ
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Molecular Weight
Approximately 35-40 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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