HDGF2 Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

HDGF2 protein actively promotes cell proliferation by enhancing DNA synthesis. This protein plays a crucial role in promoting DNA replication, an essential process required for cell division and growth. HDGF2 Protein, Human (HEK293, His) is the recombinant human-derived HDGF2 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

HDGF2 protein actively promotes cell proliferation by enhancing DNA synthesis. This protein plays a crucial role in promoting DNA replication, an essential process required for cell division and growth. HDGF2 Protein, Human (HEK293, His) is the recombinant human-derived HDGF2 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

HDGF2 protein actively promotes cell proliferation by enhancing DNA synthesis. This protein plays a crucial role in facilitating the replication of DNA, a fundamental process required for cell division and growth. By actively promoting DNA synthesis, HDGF2 protein can contribute to the regulation of cell proliferation and tissue development. Understanding the specific mechanisms by which HDGF2 protein operates can provide insights into the intricate processes that govern cell growth and division, potentially leading to advancements in our understanding of developmental biology and the identification of new therapeutic targets for diseases characterized by abnormal cell proliferation.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • HDGF2 (M1-T203)
      Accession # Q9Y3E1
    • 6*His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    HDGFL3; HDGF-2; HDGF Like 3; HDGF2; HRP-3; Hepatoma-Derived Growth Factor, Related Protein 3; Hdgfrp3; CGI-142; Hepatoma-Derived Growth Factor-Related Protein 3; HDGFRP3; Hepatoma-Derived Growth Factor 2

  • AA Sequence

    MARPRPREYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQAIQQQSSSETEGEGGNTADASSEEEGDRVEEDGKGKRKNEKAGSKRKKSYTSKKSSKQSRKSPGDEDDKDCKEEENKSSSEGGDAGNDTRNTTSDLQKTSEGT

  • Predicted Molecular Mass

    23.6 kDa

  • Molecular Weight

    Approximately 30 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM MES, 150 mM NaCl, pH 5.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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