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Hepsin/HPN Protein, Mouse (HEK293, His)

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Hepsin/HPN is a serine protease that cleaves extracellular substrates and contributes significantly to the processing of growth factors such as HGF and MST1/HGFL. It plays a vital role in cell growth and morphology maintenance. Hepsin/HPN Protein, Mouse (HEK293, His) is the recombinant mouse-derived Hepsin/HPN protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Hepsin/HPN is a serine protease that cleaves extracellular substrates and contributes significantly to the processing of growth factors such as HGF and MST1/HGFL. It plays a vital role in cell growth and morphology maintenance. Hepsin/HPN Protein, Mouse (HEK293, His) is the recombinant mouse-derived Hepsin/HPN protein, expressed by HEK293 , with C-His labeled tag.

Background

Hepsin/HPN, a serine protease, serves as a pivotal enzyme in the cleavage of extracellular substrates, contributing significantly to the proteolytic processing of growth factors like HGF and MST1/HGFL. Its involvement extends to key cellular functions, playing a crucial role in cell growth and the maintenance of cell morphology. Moreover, Hepsin/HPN is implicated in the proteolytic processing of ACE2, suggesting its participation in essential cellular pathways. Notably, this protease also mediates the cleavage of urinary UMOD, an integral step for the polymerization of UMOD, highlighting its diverse functions in modulating various biological processes.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-10*His
  • Accession

    O35453 (T60-P436)

  • Gene ID
    15451  [NCBI]
  • Molecular Construction
    • N-term
    • Hepsin (T60-P436)
      Accession # O35453
    • 10*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    HPN; Serine Protease Hepsin; Hepsin; Transmembrane Protease, Serine 1; TMPRSS1; Testicular Tissue Protein Li 85; Transmembrane Protease Serine 1; Alternative Protein HPN

  • AA Sequence

    TILLQSDQEPLYQVQLSPGDSRLAVFDKTEGTWRLLCSSRSNARVAGLGCEEMGFLRALAHSELDVRTAGANGTSGFFCVDEGGLPLAQRLLDVISVCDCPRGRFLTATCQDCGRRKLPVDRIVGGQDSSLGRWPWQVSLRYDGTHLCGGSLLSGDWVLTAAHCFPERNRVLSRWRVFAGAVARTSPHAVQLGVQAVIYHGGYLPFRDPTIDENSNDIALVHLSSSLPLTEYIQPVCLPAAGQALVDGKVCTVTGWGNTQFYGQQAMVLQEARVPIISNEVCNSPDFYGNQIKPKMFCAGYPEGGIDACQGDSGGPFVCEDSISGTSRWRLCGIVSWGTGCALARKPGVYTKVTDFREWIFKAIKTHSEASGMVTQP

  • Predicted Molecular Mass

    42.2 kDa

  • Molecular Weight

    Approximately 18-22 kDa & 30 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM NaAc,150 mM NaCl, pH 5.0, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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