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  4. Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO)

Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO)

Cat. No.: HY-P71517
Handling Instructions

Hygromycin-B kinase/HPH Proteinas, an aminoglycoside phosphotransferase, phosphorylates and inactivates antibiotic substrates, including hygromycin and related compounds. This selective enzymatic activity highlights its specificity within the aminoglycoside class, regulating antibiotic function and potentially influencing microbial resistance mechanisms. Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Hygromycin-B kinase/HPH protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO) is 341 a.a., with molecular weight of ~54.0 kDa.

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Description

Hygromycin-B kinase/HPH Proteinas, an aminoglycoside phosphotransferase, phosphorylates and inactivates antibiotic substrates, including hygromycin and related compounds. This selective enzymatic activity highlights its specificity within the aminoglycoside class, regulating antibiotic function and potentially influencing microbial resistance mechanisms. Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Hygromycin-B kinase/HPH protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO) is 341 a.a., with molecular weight of ~54.0 kDa.

Background

The Hygromycin-B kinase/HPH protein, belonging to the aminoglycoside phosphotransferases, executes the inactivation of its antibiotic substrates through phosphorylation. Specifically, it selectively phosphorylates hygromycin and closely related compounds, including demethyl analogs and destomycin. This enzymatic activity underscores the specificity of Hygromycin-B kinase/HPH in modifying its substrates within the aminoglycoside class, contributing to the regulation of antibiotic function and potentially influencing microbial resistance mechanisms.

Species

E.coli

Source

E. coli

Tag

N-His;N-SUMO

Accession

P00557 (1M-341E)

Gene ID

57286788

Molecular Construction
N-term
6*His-SUMO
HPH (1M-341E)
Accession # P00557
C-term
Synonyms
hphHygromycin-B 4-O-kinase; EC 2.7.1.163; APH(4); Hygromycin B phosphotransferase; Hygromycin-B kinase
AA Sequence

MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE

Molecular Weight

Approximately 54.0 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO)
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