Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO)

Hygromycin-B kinase/HPH Proteinas, an aminoglycoside phosphotransferase, phosphorylates and inactivates antibiotic substrates, including hygromycin and related compounds. This selective enzymatic activity highlights its specificity within the aminoglycoside class, regulating antibiotic function and potentially influencing microbial resistance mechanisms. Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Hygromycin-B kinase/HPH protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

The Hygromycin-B kinase/HPH protein, belonging to the aminoglycoside phosphotransferases, executes the inactivation of its antibiotic substrates through phosphorylation. Specifically, it selectively phosphorylates hygromycin and closely related compounds, including demethyl analogs and destomycin. This enzymatic activity underscores the specificity of Hygromycin-B kinase/HPH in modifying its substrates within the aminoglycoside class, contributing to the regulation of antibiotic function and potentially influencing microbial resistance mechanisms.

E.coli

E. coli

N-His;N-SUMO

P00557 (M1-E341)

83660448

Full Length

MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.