ICAM-1/CD54 Protein, Mouse (HEK293, His)

ICAM-1/CD54 protein is a key player in cellular interactions, acting as a ligand for LFA-1. It facilitates endothelial cup assembly during leukocyte migration and regulates immune responses. ICAM-1 also interacts with MUC1, promoting cell aggregation, and participates in immunological synapse formation between antigen-presenting cells and T cells. These interactions showcase ICAM-1's diverse role in cellular adhesion and immune modulation. ICAM-1/CD54 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-His labeled tag.

ICAM-1/CD54 protein emerges as a pivotal component in mediating cellular interactions, serving as a ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). Notably, during the trans-endothelial migration of leukocytes, ICAM-1 engagement plays a crucial role in facilitating the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. This intricate molecular process, resembling other members of the ICAM family, underscores ICAM-1's involvement in regulating immune responses and inflammatory reactions. Beyond its role in leukocyte adhesion, ICAM-1 functions as a homodimer and interacts with MUC1, promoting cell aggregation in epithelial cells. Additionally, ICAM-1 establishes interactions with ARHGEF26/SGEF and associates with CD81, CD247, and CD9 on the T cell side, contributing to the formation of immunological synapses between antigen-presenting cells and T cells. These multifaceted interactions highlight the diverse functions of ICAM-1 in cellular adhesion and immune modulation.

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM1 coated plates (12.5 μg/mL, 100 μL/well ),approximately > 40% cells will adhere specifically.

Mouse

HEK293

C-His

P13597 (Q28-N485)

15894  [NCBI]

Extracellular Domain

MASTRAKPTLPLLLALVTVVIPGPGDAQVSIHPREAFLPQGGSVQVNCSSSCKEDLSLGLETQWLKDELESGPNWKLFELSEIGEDSSPLCFENCGTVQSSASATITVYSFPESVELRPLPAWQQVGKDLTLRCHVDGGAPRTQLSAVLLRGEEILSRQPVGGHPKDPKEITFTVLASRGDHGANFSCRTELDLRPQGLALFSNVSEARSLRTFDLPATIPKLDTPDLLEVGTQQKLFCSLEGLFPASEARIYLELGGQMPTQESTNSSDSVSATALVEVTEEFDRTLPLRCVLELADQILETQRTLTVYNFSAPVLTLSQLEVSEGSQVTVKCEAHSGSKVVLLSGVEPRPPTPQVQFTLNASSEDHKRSFFCSAALEVAGKFLFKNQTLELHVLYGPRLDETDCLGNWTWQEGSQQTLKCQAWGNPSPKMTCRRKADGALLPIGVVKSVKQEMNGTYVCHAFSSHGNVTRNVYLTVLYHSQNN

80-90 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.