ICAM-1/CD54 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
ICAM-1/CD54 protein is a key player in cellular interactions, acting as a ligand for LFA-1. It facilitates endothelial cup assembly during leukocyte migration and regulates immune responses. ICAM-1 also interacts with MUC1, promoting cell aggregation, and participates in immunological synapse formation between antigen-presenting cells and T cells. These interactions showcase ICAM-1's diverse role in cellular adhesion and immune modulation. ICAM-1/CD54 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ICAM-1/CD54 protein is a key player in cellular interactions, acting as a ligand for LFA-1. It facilitates endothelial cup assembly during leukocyte migration and regulates immune responses. ICAM-1 also interacts with MUC1, promoting cell aggregation, and participates in immunological synapse formation between antigen-presenting cells and T cells. These interactions showcase ICAM-1's diverse role in cellular adhesion and immune modulation. ICAM-1/CD54 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-His labeled tag.
Background
ICAM-1/CD54 protein emerges as a pivotal component in mediating cellular interactions, serving as a ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). Notably, during the trans-endothelial migration of leukocytes, ICAM-1 engagement plays a crucial role in facilitating the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. This intricate molecular process, resembling other members of the ICAM family, underscores ICAM-1's involvement in regulating immune responses and inflammatory reactions. Beyond its role in leukocyte adhesion, ICAM-1 functions as a homodimer and interacts with MUC1, promoting cell aggregation in epithelial cells. Additionally, ICAM-1 establishes interactions with ARHGEF26/SGEF and associates with CD81, CD247, and CD9 on the T cell side, contributing to the formation of immunological synapses between antigen-presenting cells and T cells. These multifaceted interactions highlight the diverse functions of ICAM-1 in cellular adhesion and immune modulation.
Verified Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM1 coated plates (12.5 μg/mL, 100 μL/well ),approximately > 40% cells will adhere specifically.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-His
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Accession
P13597 (Q28-N485)
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Molecular Construction
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N-term
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ICAM-1 (Q28-N485)
Accession # P13597 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
ICAM1; Epididymis Secretory Sperm Binding Protein; Intercellular Adhesion Molecule 1; Intercellular Adhesion Molecule-1; CD54; Cell Surface Glycoprotein P3.58; BB2; Human Rhinovirus Receptor; Major Group Rhinovirus Receptor; CD54 Antigen; ICAM-1; P3.58; I
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AA Sequence
QVSIHPREAFLPQGGSVQVNCSSSCKEDLSLGLETQWLKDELESGPNWKLFELSEIGEDSSPLCFENCGTVQSSASATITVYSFPESVELRPLPAWQQVGKDLTLRCHVDGGAPRTQLSAVLLRGEEILSRQPVGGHPKDPKEITFTVLASRGDHGANFSCRTELDLRPQGLALFSNVSEARSLRTFDLPATIPKLDTPDLLEVGTQQKLFCSLEGLFPASEARIYLELGGQMPTQESTNSSDSVSATALVEVTEEFDRTLPLRCVLELADQILETQRTLTVYNFSAPVLTLSQLEVSEGSQVTVKCEAHSGSKVVLLSGVEPRPPTPQVQFTLNASSEDHKRSFFCSAALEVAGKFLFKNQTLELHVLYGPRLDETDCLGNWTWQEGSQQTLKCQAWGNPSPKMTCRRKADGALLPIGVVKSVKQEMNGTYVCHAFSSHGNVTRNVYLTVLYHSQNN
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Predicted Molecular Mass
51.7 kDa
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Molecular Weight
Approximately 70-90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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