ICAM-3/CD50 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The ICAM-3/CD50 protein acts as a ligand for the leukocyte adhesion protein LFA-1 and integrin α-D/β-2, suggesting involvement in cell adhesion. ICAM-3 cooperates with integrin α-L/β-2 to facilitate phagocytosis of apoptotic neutrophils, suggesting a role in immune response regulation and apoptotic cell clearance. ICAM-3/CD50 Protein, Human (HEK293, His) is the recombinant human-derived ICAM-3/CD50 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ICAM-3/CD50 protein acts as a ligand for the leukocyte adhesion protein LFA-1 and integrin α-D/β-2, suggesting involvement in cell adhesion. ICAM-3 cooperates with integrin α-L/β-2 to facilitate phagocytosis of apoptotic neutrophils, suggesting a role in immune response regulation and apoptotic cell clearance. ICAM-3/CD50 Protein, Human (HEK293, His) is the recombinant human-derived ICAM-3/CD50 protein, expressed by HEK293 , with C-His labeled tag.
Background
ICAM-3/CD50 Protein serves as a ligand for the leukocyte adhesion proteins LFA-1 (integrin alpha-L/beta-2) and integrin alpha-D/beta-2, implicating its involvement in cellular adhesion processes. When associated with integrin alpha-L/beta-2, ICAM-3 plays a contributory role in apoptotic neutrophil phagocytosis by macrophages, suggesting a potential role in the regulation of immune responses and clearance of apoptotic cells. Additionally, ICAM-3 interacts with moesin/MSN, indicating its participation in protein-protein interactions that may influence cellular signaling or structural organization. The multifaceted interactions of ICAM-3 underscore its significance in the intricate network of cellular adhesion and immune-related processes.
Verified Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated jurkatcells. When 5x104 cells/well are added to rhICAM-3/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well) in the presence of 20 ng/mL PMA, approximately 64.39% will adhere after 1 hourat 37°C.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
P32942 (Q30-H485)
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Molecular Construction
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N-term
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ICAM-3 (Q30-H485)
Accession # P32942 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
ICAM3; CD50; Intercellular Adhesion Molecule 3; ICAM-3; ICAM-R; CD50 Antigen; CDW50; CDw50
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AA Sequence
QEFLLRVEPQNPVLSAGGSLFVNCSTDCPSSEKIALETSLSKELVASGMGWAAFNLSNVTGNSRILCSVYCNGSQITGSSNITVYRLPERVELAPLPPWQPVGQNFTLRCQVEDGSPRTSLTVVLLRWEEELSRQPAVEEPAEVTATVLASRDDHGAPFSCRTELDMQPQGLGLFVNTSAPRQLRTFVLPVTPPRLVAPRFLEVETSWPVDCTLDGLFPASEAQVYLALGDQMLNATVMNHGDTLTATATATARADQEGAREIVCNVTLGGERREARENLTVFSFLGPIVNLSEPTAHEGSTVTVSCMAGARVQVTLDGVPAAAPGQPAQLQLNATESDDGRSFFCSATLEVDGEFLHRNSSVQLRVLYGPKIDRATCPQHLKWKDKTRHVLQCQARGNPYPELRCLKEGSSREVPVGIPFFVNVTHNGTYQCQASSSRGKYTLVVVMDIEAGSSH
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Molecular Weight
Approximately 100-120 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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