ICAM-3/CD50 Protein, Human (HEK293, His)

The ICAM-3/CD50 protein acts as a ligand for the leukocyte adhesion protein LFA-1 and integrin α-D/β-2, suggesting involvement in cell adhesion. ICAM-3 cooperates with integrin α-L/β-2 to facilitate phagocytosis of apoptotic neutrophils, suggesting a role in immune response regulation and apoptotic cell clearance. ICAM-3/CD50 Protein, Human (HEK293, His) is the recombinant human-derived ICAM-3/CD50 protein, expressed by HEK293 , with C-His labeled tag.

ICAM-3/CD50 Protein serves as a ligand for the leukocyte adhesion proteins LFA-1 (integrin alpha-L/beta-2) and integrin alpha-D/beta-2, implicating its involvement in cellular adhesion processes. When associated with integrin alpha-L/beta-2, ICAM-3 plays a contributory role in apoptotic neutrophil phagocytosis by macrophages, suggesting a potential role in the regulation of immune responses and clearance of apoptotic cells. Additionally, ICAM-3 interacts with moesin/MSN, indicating its participation in protein-protein interactions that may influence cellular signaling or structural organization. The multifaceted interactions of ICAM-3 underscore its significance in the intricate network of cellular adhesion and immune-related processes.

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated jurkatcells. When 5x10⁴ cells/well are added to rhICAM-3/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well) in the presence of 20 ng/mL PMA, approximately 64.39% will adhere after 1 hourat 37°C.

Human

HEK293

C-His

P32942 (Q30-H485)

3385  [NCBI]

Extracellular Domain

QEFLLRVEPQNPVLSAGGSLFVNCSTDCPSSEKIALETSLSKELVASGMGWAAFNLSNVTGNSRILCSVYCNGSQITGSSNITVYRLPERVELAPLPPWQPVGQNFTLRCQVEDGSPRTSLTVVLLRWEEELSRQPAVEEPAEVTATVLASRDDHGAPFSCRTELDMQPQGLGLFVNTSAPRQLRTFVLPVTPPRLVAPRFLEVETSWPVDCTLDGLFPASEAQVYLALGDQMLNATVMNHGDTLTATATATARADQEGAREIVCNVTLGGERREARENLTVFSFLGPIVNLSEPTAHEGSTVTVSCMAGARVQVTLDGVPAAAPGQPAQLQLNATESDDGRSFFCSATLEVDGEFLHRNSSVQLRVLYGPKIDRATCPQHLKWKDKTRHVLQCQARGNPYPELRCLKEGSSREVPVGIPFFVNVTHNGTYQCQASSSRGKYTLVVVMDIEAGSSH

100-120 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.