1. Protéines recombinantes
  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-alpha
  5. IFN-alpha 2a
  6. IFN-alpha 2/IFNA2 Protein, Mouse (HEK293, hFc)

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  • Activité biologique

  • Paramètres Techniques

  • Propriétés

  • Description

Activité biologique

Measured by its binding ability in a functional ELISA. Immobilized Mouse IFNAR1 at 2 μg/ml (100 μL/well) can bind Mouse IFNA2. The ED50 for this effect is 73.44 ng/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized Mouse IFNAR1 at 2 μg/mL (100 μL/well) can bind Mouse IFNA2. The ED50 for this effect is 73.44 ng/mL.
Species

Mouse

Source

HEK293

Tag

N-hFc

Accession

P01573 (C24-E190)

Gene ID

15965

Molecular Construction
N-term
hFc
IFNA2 (C24-E190)
Accession # P01573
C-term
Protein Length

Full Length of Mature Protein

Synonyms
Interferon alpha-2; IFN-alpha-2; LeIF A; IFNA2; IFNA2A
AA Sequence

CDLPHTYNLRNKRALKVLAQMRRLPFLSCLKDRQDFGFPLEKVDNQQIQKAQAIPVLRDLTQQTLNLFTSKASSAAWNATLLDSFCNDLHQQLNDLQTCLMQQVGVQEPPLTQEDALLAVRKYFHRITVYLREKKHSPCAWEVVRAEVWRALSSSVNLLPRLSEEKE

Predicted Molecular Mass
45.4 kDa
Masse moléculaire

Approximately 48-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Glycosylation
Yes
Pureté
  • ≥ 95%, as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Livraison

Room temperature in continental US; may vary elsewhere.

Documentation

IFN-alpha 2/IFNA2 Protein, Mouse (HEK293, hFc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Nom du produit:
IFN-alpha 2/IFNA2 Protein, Mouse (HEK293, hFc)
Cat. No.:
HY-P73248A
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MCE Japan Authorized Agent: