IL-12 Protein, Human (Biotinylated, HEK293, His-Avi, Flag)
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-His;C-Flag
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Accession
P29459 (R23-S219)&P29460 (I23-S328)
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Gene ID3592&3593
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Molecular Construction
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N-term
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IL12A (R23-S219)
Accession # P29459 -
Flag
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C-term
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N-term
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IL12B (I23-S328)
Accession # P29460 -
His-Avi
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C-term
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Conjugation
Biotin
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Conjugate Method
The single lysine residue in Avitag is biotinylated through an enzymatic reaction.
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Synonyms
IL12A; Cytotoxic Lymphocyte Maturation Factor 1, P35; Prev. NKSF1; NK Cell Stimulatory Factor Chain 1; IL-12A; Interleukin-12 Alpha Chain; Interleukin-12 Subunit Alpha; Interleukin 12, P35; CLMF; IL-12, Subunit P35; NFSK; IL35 Subunit; P35; CLMF P35; Inte
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Predicted Molecular Mass
38.3 kDa (IL-12B) & 23.8 kDa (IL-12A)
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Molecular Weight
Approximately 32-35 kDa (IL12A) & 45-50 kDa (IL12B), based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)